直肠末端平滑肌组织对铜离子反应的实验研究  

The tissue reaction induced by copper ions to the smooth muscle of the terminal rectum

作　　者：常宝志[1] 黄莚庭[1] 那加[2] 林志彬[3] 胡章顺[1] 李卫东[3] 满蒙[4] 

机构地区：[1]北京大学第一医院外科,100034 [2]北京大学第一医院病理科,100034 [3]北京大学基础医学院药理教研组 [4]北京计然电子科技有限公司

出　　处：《中华普通外科杂志》2005年第3期177-179,共3页Chinese Journal of General Surgery

摘　　要：目的　观察铜离子对直肠末端平滑肌组织的刺激反应。方法　选用雄性家兔6 5只,用铜离子电化学治疗仪的铜针刺入直肠末端齿状线上方的黏膜下平滑肌组织内,随机按实验操作后1、3、4、6、8周的不同时间分为5个组,每组10只,观察组织学变化。结果　各组针刺部位外观均未发现黏膜有异常表现。病理学所见:1周组黏膜下轻度水肿,8只炎细胞浸润明显,主要是浆细胞和淋巴细胞,2只浸润为中度。3周组炎症减轻。4周组仍有轻度炎症,5周时炎症消失。各组平滑肌组织均正常。另用铂针和钢针做对照,观察铜针对组织是否有特殊反应,每组5只,10只有轻度浆细胞浸润,而中性白细胞浸润明显,有溃疡、小脓肿形成及炎性坏死。钢针组尤为明显。结论　铜针刺入组织可产生炎症,5周后完全消退,其特点是以浆细胞和淋巴细胞浸润为主,可能有利于加强组织局部的抗感染能力,但对平滑肌组织无损害。钢、铂针的组织损害则比较严重。Objective To investigate the tissue stimulation of copper ions to the smooth muscle of murine terminal rectum.Methods In this study copper needle used for electrochemical therapeutic equipment was punctured into submucosal smooth muscle tissue of terminal rectum above the dentate line in 65 male rats.Rats were then divided into 5 groups (10 rats in each group) at random and sacrificed at 1, 3, 4, 6, 8 weeks after respectively, samples were sent for observation of macroscopic and microscopic tissue reaction.Results Rats had no abnormal histological change in the puncture points grossly. Undor microscope, mild edema was detected in the submucosal layer in 1 week group. Remarkable infiltration of inflammatory cells and lymphocytes subsided 3 weeks after, and disappeared five weeks after.Smooth muscle was normal microscopically in all groups.In contrast, platinum and steel needles caused infiltration of plasma cells and neutrophils, ulcers and small abscess formation in around puncture points.Conclusion Inflammatory reaction was induced after puncturing of copper needle into the tissues and the tissue reaction disappeared after 5 Ws.In contrast with platinum and steel needles, copper needle was non-traumatic to the smooth muscle tissue.

关 键 词：平滑肌组织 铜离子 实验研究 直肠 电化学治疗仪 淋巴细胞浸润 组织学变化 炎细胞浸润 浆细胞浸润 白细胞浸润 抗感染能力 刺激反应 雄性家兔 实验操作 不同时间 异常表现 针刺部位 特殊反应 炎性坏死 脓肿形成 组织损害 黏膜下 炎症 离子对 组织内 齿状线 铜针 

分 类 号：R657.1[医药卫生—外科学]