不同营养途径对腹腔感染大鼠肠道微生态的影响  

Influences of enteral and parenteral nutrition on the gut microecology of the rats with abdominal infection

作　　者：沈通一[1] 秦环龙[1] 高志光[1] 范小兵[2] 杭晓敏[2] 蒋燕群[3] 

机构地区：[1]上海交通大学第六人民医院外科,上海200233 [2]上海交通大学昂立生物医学研究所 [3]上海交通大学第六人民医院检验科,上海200233

出　　处：《肠外与肠内营养》2005年第2期69-71,75,共4页Parenteral & Enteral Nutrition

基　　金：国家自然科学基金资助项目(批准号: 30471687)

摘　　要：目的:探讨肠内肠外营养对腹腔感染大鼠肠道微生态的影响。　方法:将 14只存活超过 6天的盲肠结扎穿孔腹腔感染大鼠,分别给予肠外营养(PN组,n=7)和PN+肠内营养(EN组,n=7)。两组动物营养供给等热量、等氮量。第 6天处死,取盲肠内容物做厌氧培养,采用随机扩增多态性DNA技术(RAPD)作细菌种群DNA指纹图谱分析。　结果:①实验动物总体病死率为 69. 6%,大多数动物出现腹腔脓肿、肝脓肿、肺部感染等;②PN组各菌种数量减少,EN组各菌种数量增多,但条件致病菌(如产气荚膜杆菌 )数量也增加;③细菌DNA指纹图谱显示,PN组肠道菌群条带减少,而且出现异常条带;EN组同正常大鼠有较高的一致性。　结论:早期肠内营养能克服单纯PN导致的菌群破坏,使肠道菌群数量增多,但菌种上缺乏控制,可导致个别条件致病菌增加。Objective: To investigate the influences of enteral and parenteral nutrition suppled by gut on the gut microecology of the rats with abdominal infection. Methods: 14 Sprague-Dawley(SD)rats with abdominal infection models made with cecal ligation and perforation method were divided into two groups: parenteral nutrition (PN group, n=7) and PN+enteral nutrition (EN group, n=7) . The components of nutrition solutions in the two groups were isonitrogen and isocaloric. The rats were killed in the sixth day. The feces in cecum were cultured for anaerobic bacterial growth and analysed by bacterial group DNA fingerprint spectrum with random amplified polymorphic DNA . Results: ①The total death rate of the rats was 69.6%. Abdominal cavity abscess, liver abscess, and pulmonary infection commonly appeared in the model rats. ②The number of germs cultured in EN group was higher than in the PN group, but the quantity of Cl Perfrigens was higher , too. The gene stripe of probiotics group in EN group was similar to that in normal rats showed in the DNA fingerprint spectrum, but was significantly different in PN group with strange stripes. Conclusion: Application of early EN may correct the intestinal flora disturbance resulted from PN and increase the quantity of intestinal flora, but it can't control the species and may increase some conditional pathogenic bacteria .

关 键 词：腹腔感染 PN 大鼠 EN 肠道微生态 营养途径 肠外营养 种数 种群 厌氧培养 

分 类 号：R459.3[医药卫生—治疗学] R656[医药卫生—临床医学]