大鼠胰腺移植急性排斥反应中调节激活正常T细胞表达和分泌因子的作用  

Role of RANTES in the acute rejection of pancreas transplantation in rats

作　　者：朱军[1] 徐泽宽[1] 苗毅[1] 

机构地区：[1]南京医科大学第一附属医院胰腺外科,210029

出　　处：《中华实验外科杂志》2005年第4期410-412,共3页Chinese Journal of Experimental Surgery

基　　金：江苏省卫生厅 135重点人才基金资助项目 (苏卫科教[2 0 0 3] 1 9号 )

摘　　要：目的　探讨在大鼠胰腺移植急性排斥反应中调节激活正常T细胞表达和分泌因子(RANTES)的作用。方法　对链脲佐菌素诱导的糖尿病大鼠模型施行胰腺移植。术后1、4、7、10d4个时间点取材,采用酶联免疫吸附法检测受体血清RANTES的浓度,免疫组织化学法测定移植胰腺组织RANTES蛋白表达水平。结果　急性排斥反应在术后7d达高峰。术后1、4、7、10d受体血清RANTES浓度在同系移植组分别为(3 5 7.87±63 .2 6)、(2 74.77±5 8.2 2 )、(2 65 .87±43 .40 )、(2 67.5 5±48.91)ng/L ,除1d外与对照组比较差异无统计学意义(2 5 1.18±44 .94)ng/L ;在异系移植组分别为(3 88.48±10 4.45 )、(5 86.72±90 .0 6)、(746.2 8±92 .64 )、(63 1.49±10 6.3 4)ng/L ,与对照组比较差异有统计学意义(P <0 .0 5 )。移植胰腺组织RANTES蛋白的表达强度在同系移植组中与对照组比较无显著变化,而在异系移植组随排斥反应的发展呈动态变化,在4d为中度阳性,至7d表达为强阳性。结论　胰腺移植急性排斥反应过程与RANTES的表达密切联系。Objective To investigate the role of regulated upon activation,normal T cell expressed and secreted (RANTES) in the acute pancreatic allograft rejection in rats.Methods Diabetic SD rats were induced by streptozocin (STZ) firstly and then underwent pancreas transplantation by a physiologic method of portal venous and enteric drainage.The serum RANTES was assessed by ELISA and the expression of RANTES in the pancreas grafts was detected by immunohistochemistry dynamically at 1,4,7,10 day post transplantation.Results The concentration of serum RANTES was 357.87± 63.26, 274.77± 58.22, 265.87± 43.40, 267.55± 48.91 respectively at 1,4,7,10 day post transplantation in the allograft group,which had no significant difference compared to the control group (251.18± 44.94) ng/L except for 1 day.The concentration of serum TANTES was (388.48± 104.45), (586.72± 90.06), (746.28± 92.64), (631.49± 106.34) ng/L respectively in the isograft group,which had significant difference compared to the control group ( P < 0.05). The expression of RANTES in the pancreas allografts appeared moderately at 4 day and strongly at 7 day,which was relative with the development of acute rejection,though it was not observed in the isografts.Conclusion RANTES is involved in process of acute rejection.Detection of RANTES may be useful in the diagnosis of acute rejection in pancreas transplantation.

关 键 词：急性排斥反应 胰腺移植 T细胞表达 分泌因子 激活 调节 RANTES 酶联免疫吸附法 免疫组织化学法 蛋白表达水平 受体血清 胰腺组织 同系移植 链脲佐菌素 对照组 大鼠模型 动态变化 反应过程 早期诊断 动态检测 移植术后 统计学 糖尿病 S蛋白 强阳性 浓度 组分 

分 类 号：R657.5[医药卫生—外科学]