以血管内皮生长因子受体为靶点抑制瘢痕血管增生的研究  

Angiogenesis suppressed in hypertrophic scar by vascular endothelial growth factor receptor-2-targeted therapy

作　　者：祁少海[1] 沈锐[1] 谢举临[1] 刘坡[1] 徐盈斌[1] 利天增[1] 

机构地区：[1]中山大学附属第一医院烧伤科,广州510080

出　　处：《中华实验外科杂志》2005年第4期421-423,i001,共4页Chinese Journal of Experimental Surgery

基　　金：广东省重点科技攻关项目 (982 781 72 )

摘　　要：目的　以血管内皮生长因子受体 2 (VEGFR 2 /KDR)为靶点,观察其抗体对烧伤增生性瘢痕新生血管形成的作用。方法　以人烧伤增生性瘢痕移植于裸鼠建立动物模型。治疗组KDR多抗分为10mg/L和5mg/L两组,以磷酸盐缓冲液(PBS)组和空白对照组作对照,每周2次,治疗1、2、3周分别观察瘢痕体积、组织形态学、微血管定量及Ⅰ、Ⅲ型胶原含量的变化。结果　治疗3周后的瘢痕体积(mm3 )、血管密度(个/mm2 )、Ⅰ、Ⅲ型胶原含量(阳性面积) ,KDR两治疗组均与对照组差异有统计学意义(P <0 .0 5 )。形态学显示瘢痕组织内有大量的血管内皮细胞坏死和血管闭塞,成纤维细胞凋亡,对照组变化不明显。Objective To investigate the angiogenesissuppressed by vascular endothelial growth factor receptor-2 (VEGFR-2/KDR)-targeted therapy in post-burn hypertrophic scar.Methods A hypertrophic scar animal model was made.Intralesional injection of Kinase insert domain containing receptor (KDR) polyclonal antibody (10 mg/L and 5 mg/L) was performed for 3 weeks (twice one week).There were two control groups:PBS group and blank group.The changes of scar in volume and morphology were observed on the first,second and third week after injection.Results The volume of scar,the density of capillary,and the amount of collagen I and collagen Ⅲ in KDR polyclonal antibody groups were less than those in the control groups significantly ( P < 0.05). The morphology examinations demonstrated many dead or apoptotic fibroblasts and endothelial cells in treatment groups.Conclusion KDR antibody can be used to cure hypertrophic scar by suppressing angiogenesis.

关 键 词：血管内皮生长因子受体 血管增生 抑制 靶点 烧伤增生性瘢痕 成纤维细胞凋亡 新生血管形成 磷酸盐缓冲液 胶原含量 组织形态学 微血管定量 KDR 对照组 动物模型 瘢痕移植 血管密度 血管闭塞 细胞坏死 治疗组 统计学 组织内 抗体 体积 Ⅲ型 

分 类 号：R622[医药卫生—整形外科]