急性有机磷农药中毒大鼠骨骼肌组织ATP酶活性的变化及硫酸镁对其影响  

ATPase activities in skelet muscle tissue after acute organophosphorus poisoning and the effects of magnesium sulfate

作　　者：李长喻[1] 赵敏[1] 杨柳[2] 贾兰玲[3] 

机构地区：[1]中国医科大学附属第二医院急诊科,沈阳110004 [2]新民市人民医院循环科 [3]中国医科大学附属第二医院肿瘤研究所,沈阳110004

出　　处：《中华急诊医学杂志》2005年第4期276-278,共3页Chinese Journal of Emergency Medicine

摘　　要：目的探讨急性有机磷农药中毒大鼠骨骼肌组织ATP酶活性变化及硫酸镁对其影响。方法30只健康大鼠随机分成3组,每组10只,分别为正常对照组(A组);氧化乐果染毒组(B组);硫酸镁预处理后再染毒组(C组)。测定3组大鼠骨骼肌组织Na+K+ATP酶和Ca2+ATP酶活性,并进行组间比较。结果与A组相比,B组Na+K+ATP酶,Ca2+ATP酶活性均明显降低(P<0.01)。与B组相比,C组Na+K+ATP酶和Ca2+ATP酶活性均有不同程度地恢复,Ca2+ATP酶活性恢复(P<0.01)较Na+K+ATP酶活性恢复(P<0.05)明显。结论急性有机磷农药中毒后,骨骼肌组织Na+K+ATP酶,Ca2+ATP酶活性均明显受抑制。硫酸镁预处理能减轻ATP酶活性的受抑制程度,尤其是对Ca2+ATP酶。Objective To investigate the ATPase activities of skelet muscle tissue in rats with acute organophosphorus poisoning,and to explore the effects of magnesium sulfate on ATPase activities.Methods Thirty healthy rats were randomly divided into three groups:group A(control),group B(oxide dimethoate given intraperitoneally),and group C(magnesium sulfate administrated before oxide dimethoate).The activities of Na +-K +-ATPase and Ca 2+-ATPsae of skelet muscle tissue were measured respectively.Comparisons were made among the three groups.Results The activities of Na +-K +-ATPase and Ca 2+-ATPase were decreased obviously in group B as compared with group A(P<0.01). The activities of Na +-K +-ATPase and Ca 2+-ATPase recoverd at different degrees in group C as compared with group B. The activities of Ca 2+-ATPase recovered more obviously (P< 0.01 ) than Na +-K +-ATPase activities(P< 0.05 ) .Conclusion The activities of Na +-K +-ATPase and Ca 2+-ATPase of skelet muscle tissue are inhibited when rats is given oxide dimethoate. Magnesium sulfate preparation can reduce the inhibition on ATPase, especially on Ca 2+-ATPase.

关 键 词：急性有机磷农药中毒 骨骼肌组织 CA^2+-ATP酶活性 NA^+-K^+-ATP酶 硫酸镁 活性变化 健康大鼠 氧化乐果 不同程度 预处理 对照组 B组 恢复 中毒后 染毒 C组 相比 抑制 

分 类 号：R595.4[医药卫生—内科学] R826.5[医药卫生—临床医学]