家蚕杂交分离系间的分子标记特征研究  

The Character of Molecular Markers Between Segregation Lines of Hybridization in Silkworm Bombyx mori

作　　者：司马杨虎[1,2] 陈大霞[1] 孙德斌[1] 何斯美[3] 钱荷英[3] 李斌[1] 鲁成[1] 向仲怀[1] 

机构地区：[1]农业部蚕桑学重点开放实验室,西南农业大学蚕学与生物技术学院,重庆400716 [2]苏州大学农业科学与技术学院,镇江212018 [3]中国农业科学院蚕业研究所

出　　处：《蚕业科学》2005年第1期36-41,共6页ACTA SERICOLOGICA SINICA

基　　金：国家863项目(编号2002AA211011);国家自然科学基金重点项目(编号30170719)。

摘　　要：以两个日系家蚕品种“湘晖”(P1)和“872”(P2)为亲本进行杂交,从F1自交的F2代开始,分别向茧丝量高和低两个方向进行选择。经过F2-F7共6代的同蛾区交配选择,获得了茧丝量性状有显著差异的A、C两个家系。用5组引物扩增所获得的617个AFLP分子标记,分析了A、C两个杂交分离系的分子标记特征及其DNA多态性。结果显示P1能将更多的标记传给C系,亲本P2能将更多的标记传给A系,A系保留了更多的两亲共有的标记;A系含较稳定的特异性标记23个,C系含特异性标记7个,推测A系的这些特异性标记与家蚕某些经济性状有关;通过杂交分离系各世代的遗传距离和聚类分析,F6代同一家系内差异更小,A系与C系家系间的差异更加明显,即同蛾区交配到F6代系统就基本稳定。With the Japanese line “Xianghui”and “872” as the parents, the offspring after F_2 was selected according to high (A line) or low (C line) cocoon silk amount respectively. Two strains A and C, which have significant differences in cocoon silk trait, were obtained by selection from F_2 to F_7. 617 molecular markers were obtained by AFLP from 5 primers. The molecular marker and DNA polymorphisms of two strains were analyzed. The results showed that C strain tends to like P_1, while A strain tends to like P_2, and that A strain has more common markers with parents. A strain had 23 specific markers, and C strain had 7 specific markers. The specific markers of A strain maybe relative to some economic quantitive traits of silkworm. The dendrogram by UPGMA suggested that it is less diversity among the individuals of F_6 generation of same strain, and more diversity between different strain. Which suggested that the strain is stable in heredity while mating is within the same batch till F_6 generation.

关 键 词：家蚕 近亲交配 选择 分子标记 

分 类 号：S881.2[农业科学—特种经济动物饲养]