家蚕伴性赤蚁sch胚胎期致死特异蛋白差异的研究  

Study on Special Proteins of Embryo Lethality of sch Silkworm,Bombyx mori

作　　者：卢忠燕[1,2] 侯勇[1] 赵萍[1] 林英[1] 夏庆友[1] 

机构地区：[1]西南农业大学蚕学与生物技术学院,农业部蚕桑学重点开放实验室,重庆400716 [2]第三军医大学基础部

出　　处：《蚕业科学》2005年第1期42-46,共5页ACTA SERICOLOGICA SINICA

基　　金：教育部重点项目(编号204120);国家自然科学基金项目(编号30330460;30270691)。

摘　　要：利用双向电泳技术分析了家蚕生态致死突变伴性赤蚁sch和正常黑蚁夏芳在常温常湿、高温干燥催青条件下的蚕卵差异表达蛋白质,发现高温敏感伴性致死赤蚁的蚕卵在高温催青处理后,一个分子量50kD左右的蛋白点P34与正常相比有明显差异,高温干燥处理后该蛋白点浓度较弱,表达量较低;对照黑蚁夏芳的P34蛋白没有明显差异。对P34进行胰蛋白酶消化并测定了其肽质量指纹图谱,通过同源性比较分析发现P34蛋白是一种新的蛋白质。In order to illustrate the mechanism of embryo lethality of sch,the proteins of sch silkworm egg incubated under normal condition and under high temperature and humidity are analyzed by two-dimensional gel electrophoresis.The result showed that the concentration of P3-4 protein of sch eggs incubated under different conditions is obviously different.The concentration of P3-4 of sch eggs incubated under high temperature and humidity was lower than that under normal condition.However,the control Xiafang have no difference under different conditions.Analyzed by matrix-assisted laser adsorption ionization time of flight mass spectrometry and searched silkworm database,the P3-4 may be a new protein.

关 键 词：双向电泳 肽质量指纹图谱 伴性赤蚁(sch) 差异蛋白 

分 类 号：S881.2[农业科学—特种经济动物饲养] Q51[农业科学—畜牧兽医]