模拟缺血预适应对原代培养乳鼠窦房结细胞内钙及L-型钙电流的影响  

Effects of mimic ischemic preconditioning on [Ca^(2+)]_i and L-type calcium current of cultured sinoatrial node cells of neonatal rat

作　　者：张倩[1] 宋治远[1] 仝识非[1] 黄骥[1] 

机构地区：[1]第三军医大学西南医院心血管内科

出　　处：《第三军医大学学报》2005年第6期494-496,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(30070314)~~

摘　　要：目的　观察模拟缺血预适应(IP)对培养乳鼠窦房结细胞内Ca2+及I -LCa的影响,探讨IP对窦房结细胞的保 护机制。方法　取培养2d的乳鼠窦房结细胞,随机分为:①对照组;②模拟缺血 再灌注(I R)组;③模拟IP组。以免疫荧 光技术标记细胞内Ca2+,通过激光共聚焦显微镜检测荧光强度变化;以全细胞膜片钳技术检测I -LCa。结果　模拟IP能显 著降低I R后窦房结细胞内Ca2+荧光强度,增加相应电压下的I- LCa峰值,使电流 电压曲线相对下移。结论　模拟IP能 减轻I R引起的窦房结细胞内Ca2+超载及I -LCa抑制。Objective To study the effects of mimic ischemic preconditioning (IP) on i and I-LCa of sinoatrial node cells and explore the protective effects of IP. Methods Cells were randomized to three groups: control, ischemia/reperfusion (I/R), IP. After labeled with fura4, fluorescence intensity of i was studied with laser confocal microscopy and I-LCa was recorded by whole-cell patch clamp technology. Results IP significantly reduced the fluorescence intensity of i and enhanced the peak of I-LCa as compared with I/R, shifted the I-V curve to more negative value. Conclusion IP can reduce the overload of i caused by I/R and increase I-LCa weakened by I/R.

关 键 词：窦房结细胞 缺血预适应 钙离子 L-型钙电流 

分 类 号：R322.11[医药卫生—人体解剖和组织胚胎学] R329.27[医药卫生—基础医学]