Separase上调对肿瘤细胞染色体分离作用的研究及条件表达载体的构建  

Effects of up-regulation of separase/esp1 on tumor chromatid separation and construction of a conditional expression vector

作　　者：由莉越[1] 刘昕[1] 

机构地区：[1]第三军医大学基础医学部分子遗传学教研室,重庆400038

出　　处：《第三军医大学学报》2005年第6期497-499,共3页Journal of Third Military Medical University

摘　　要：目的　研究esp1在肺腺癌细胞株spc细胞中对染色体分离的作用,为肿瘤的基因治疗探索新的方法。方法 　将已有的含esp1基因的EGFP N1质粒转染spc细胞,检测其转染效率及对染色体分裂相的影响。同时构建能依赖四环 素调控的含有esp1基因的pTRE- d2EGFP质粒载体。结果　表达载体EGFP N1 esp1转染成功,染色体异常分裂相减少。表 达载体pTRE -d2EGFP esp1构建成功。结论　esp1的上调表达对于肿瘤的异常染色体分裂相有一定的抑制作用,对于肿瘤 治疗有潜在的应用价值。Objective To construct an expression vector containing esp1 in order to investigate the effects on the chromatid separation. Methods A conditional plasmid vector containing esp1 was constructed, and was transfected into spc cells. The expression level of esp1 was detected by fluorescence microscopy, and the chromosomes were observed. Results EGFP-N1-esp1 was expressed in spc cells, and improper chromosome segregation was reduced. The plasmid of pTRE-d2EGFP-esp1 was constructed successfully. Conclusion Up-regulation of esp1 has inhibitory effects on the abnormal chromatid separation of tumors and may be potential in the treatment of cancer.

关 键 词：spe细胞 esp1 转染 四环素 

分 类 号：R394.33[医药卫生—医学遗传学] R394.2[医药卫生—基础医学]