大鼠胚胎视杯干细胞的分布研究  

Distribution of stem cells of optic cup in rat embryos

作　　者：黄小勇[1] 阴正勤[1] 王仕军[1] 曾玉晓[1] 

机构地区：[1]第三军医大学西南医院全军眼科中心,重庆400038

出　　处：《第三军医大学学报》2005年第6期504-506,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(30270457)~~

摘　　要：目的　探索视杯干细胞在大鼠胚胎视杯内的分布及分化特点。方法　对胚龄11～15d的大鼠胚胎视杯组 织做连续水平位冰冻切片,特异的抗CHX10及视网膜细胞标志蛋白免疫组化染色。结果　12.5d龄胚胎的CHX10阳性细 胞主要集中视杯的内外层,多呈复层上皮样排列,在视杯边缘层呈簇状分布;13.5d龄胚胎中,视杯外层细胞内出现色素, 内层玻璃体侧细胞出现Thy1.1阳性细胞,14.5d龄,内层Thy1.1阳性细胞明显增加。结论　视杯干细胞分布在胚胎视杯 内外层和边缘层,12.5d龄胚胎的视杯中干细胞丰富,未见分化细胞出现。Objective To study the location of embryonic optic cup stem cells during tailbud stage. Methods The embryonic optic cup at embryonic day 11～15 (E11～15) in rats was sectioned horizontally at 15 μm thick. The distributive characteristics of embryonic optic cup progenitor cells were revealed by immunohistochemistry. Results ①The distribution of optic cup progenitors was mainly aggregated on the optic cup at E12.5. CHX10-positive cells were organized as stratified epithelium arrangement on optic cup inner layer. Clusters of CHX10-positive cells were observed at the edge of optic cup; ② Pigment appeared in the outer layer of optic cup at E13.5, and differentiation into ganglion cells was initiated. Conclusion The distribution of optic cup stem cells is mainly aggregated on the optic cup at E12.5 in which the differentiation into ganglion cells is not initiated.

关 键 词：胚胎 视杯干细胞 分布 

分 类 号：R322.91[医药卫生—人体解剖和组织胚胎学] R329.1[医药卫生—基础医学]