人参多糖对K562细胞增殖抑制及诱导分化的实验研究  

Effects of Ginseng polysaccharide on proliferation and differentiation of K562 cells

作　　者：陈地龙[1] 刘永刚[1] 王亚平[1] 陈婷梅[1] 郑敏[1] 姜蓉[1] 

机构地区：[1]重庆医科大学基础医学院组织胚胎学教研室

出　　处：《第三军医大学学报》2005年第6期517-520,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(39670880);重庆市教委资助课题(2002)~~

摘　　要：目的　研究人参多糖(GPS)对人白血病细胞株(K562)增殖抑制及诱导分化的影响。方法　采用细胞体外培 养技术,MTT法检测细胞增殖抑制,光镜和电镜观察细胞形态变化;联苯胺(Benzidine)、糖原染色(PAS)、过氧化物酶 (POX)、非特异性脂酶(α- NAE)细胞化学染色分别检测K562细胞向红系、粒系、巨核系分化的特征;免疫细胞化学检测细胞 表面分化抗原及因子表达(CD14、CD15、HIR2、EPO、GM CSF);FAS及FAS L等检测细胞凋亡情况。结果　GPS对K562细胞 的增殖有明显抑制作用并能诱导K562细胞向红系或粒系细胞分化,表现为:①K562细胞的增殖受到抑制;②K562细胞形 态学上可见细胞体积缩小,核直径减小,核仁减少或消失,核染色质浓缩,胞浆丰富,核浆比例降低,可见中晚期粒、红系细 胞;③K562细胞血红蛋白(Hb)、糖原、α- NAE的表达明显增加,而过氧化物酶(POX)的表达与对照无显著性差异;④细胞表 面分化抗原CD15、HIR2、EPO、GM CSF的表达明显增强,CD14的表达与对照无显著性差异;⑤细胞凋亡明显增加。结论　人 参多糖(GPS)能明显抑制K562细胞增殖并能诱导其凋亡并使K562细胞向成熟方向分化。Objective To observe the effects of ginseng polysaccharide (GPS) on the proliferation and differentiation of K562 cells. Methods In vitro cell culture, MTT, morphological observation, and cytochemistry were used to observe the features of erythroid, granulocytic, and megakaryocytic lineage differentiated from K562 cells. We used Fas and Fas-L to observe the apoptosis of K562 cells. Results GPS could significantly inhibit the proliferation and promote differentiation of K562 cells into the granulocytes or erythrocyte in vitro: ① The proliferation of K562 cells was inhibited; ② The morphological feature of K562 cells tended to be more mature; ③ GPS could remarkably promote the expressions of hemoglobin, PAS, and α-NAE in K562 cells; ④ The expressions of CD 14, CD 15, HIR 2, EPO, and GM-CSF increased in K562 cells treated with GPS; ⑤ The apoptosis of K562 cells treated with GPS increased significantly. Conclusion GPS can restrain the proliferation and promote differentiation of K562 cells.

关 键 词：人参多糖 K562 增殖抑制 诱导分化 

分 类 号：R282.71[医药卫生—中药学] R73-354[医药卫生—中医学]