成都汉族群体15个短串联重复序列基因座遗传多态性  

Genetic polymorphisms of fifteen short tandem repeat loci in Chengdu Han population

作　　者：李英碧[1] 吴谨[1] 侯一平[1] 张霁[1] 廖淼[1] 张林[1] 陈国弟[1] 

机构地区：[1]四川大学华西基础医学与法医学院,成都610041

出　　处：《中华医学遗传学杂志》2005年第2期169-173,共5页Chinese Journal of Medical Genetics

基　　金：四川省科技厅应用基础项目基金(03JY029-074-1);美国纽约中华医学基金会基金(2000-636);教育部科技基础平台项目(505015)~~

摘　　要：目的　获得15个短串联重复(short tandem repeat,STR)基因座在成都汉族群体的群体遗传学数据。方法　2 10份EDTA抗凝血样采自成都地区无血缘关系的汉族个体。Chelex法提取DNA,PCR复合扩增,自动基因分析仪电泳收集电泳结果数据,基因扫描分析软件计算扩增产物片段相对大小,基因分型软件进行样本基因型分型。结果　全部样本的每个STR基因座都获得了清晰的基因型分型结果。15个STR基因座的杂合度介于0 .5 2 9～0 .881之间。累计非父排除率和累计个人识别机率为0 .999998和7.3×10 - 1 7。结论　经一次扩增电泳可获得15个STR基因座的基因型分型结果并明确样本性别。累计非父排除率和累计个人识别机率较高,适用于法医学亲权鉴定和个人识别。Objective To acquire the population genetic data of fifteen short tandem repeat(STR) loci in Chengdu Han population. Methods A total of 210 EDTA-blood specimens were collected from the unrelated individuals in Chengdu Han population. The DNA samples were extracted with Chelex method and amplified by multiplex PCR technique. The PCR products were analyzed by an automatic genetic analyzer; the relative fragment's lengths of PCR products were calculated by gene scan analysis software and afterward genotyped by genotype software. Results Fifteen STR loci of the 210 samples showed a successful result of genotyping. The heterozygosities of the fifteen STR loci in Chengdu Han population were found to be 0 529-0.881; the combined exclusion probability and discrimination power for the fifteen STR loci in Chengdu Han population were determined to be 0 999998 and 7.3×10 -17 respectively. Conclusion The distinct genotype of fifteen STR loci and the sex of sample could be unveiled just through PCR and electrophoresis once, and a higher measured value could be obtained for both the combined discrimination power and the exclusion probability; the fifteen STR loci can meet the needs of the parentage testing and personal identification in forensic medicine.

关 键 词：汉族群体 短串联重复序列 遗传多态性 STR基因座 PCR复合扩增 非父排除率 EDTA抗凝 Chelex 个人识别 分型结果 群体遗传学 无血缘关系 基因型分型 成都地区 基因分型 扩增产物 分析软件 基因扫描 亲权鉴定 DNA 电泳 分析仪 

分 类 号：Q987[生物学—遗传学]