西藏珞巴族HLA-A,-B基因多态性研究  

Determination of HLA-A,-B allele polymorphism in the Luoba nationality living in Tibet Autonomous Region in China

作　　者：康龙丽[1] 张洪波[1] 高放[1] 袁东亚[2] 邓天吉[3] 阎春城[3] 李生斌[1] 

机构地区：[1]西安交通大学教育部环境与疾病相关基因重点实验室,咸阳西藏民族学院医学系710061 [2]西藏民族学院医学系 [3]西藏林芝中国人民解放军115医院

出　　处：《中华医学遗传学杂志》2005年第2期227-228,共2页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金(39970401)~~

摘　　要：目的　调查西藏地区珞巴族群体HL A- A,- B基因的多态性。方法　用聚合酶链反应-序列特异性寡核苷酸探针反向斑点杂交技术,对西藏林芝地区3代内无血缘关系的92个珞巴族健康个体进行了HL A- A,- B位点的基因分型。结果　在HL A- A位点共检出10种等位基因,在HL A- B位点检出19种等位基因;在HL A- A位点高频等位基因是HL A- A* 11、- A* 0 2、- A* 2 4 ,它们的频率分别为36 .4 0 %、2 5 .5 0 %、2 3.90 % ,这3种等位基因共占珞巴族可检出等位基因的85 .80 %。在HL A- B位点高频基因为HL A- B* 4 0 (频率为2 7.2 0 % )、- B* 15 (11.4 0 % )和- B* 38(10 .90 % ) ,它们占等位基因的4 9.5 %。结论　珞巴族与其他各华人群体间都存在较大的差异,显示其HL A等位基因频率分布的民族独特性;但其HL A- A、- B等位基因多态性与藏族的很接近,这与民族学、历史学和社会学研究结果相一致。Objective To investigate the HLA-A, -B allele polymorphism in the Luoba ethnic population. Methods HLA-A,-B DNA types in 92 healthy individuals of Luoba nationality in the Linzhi area, Tibet Autonomous Region, were investigated by polymerase chain reaction-sequence specific oligo-nucleotide (PCR-SSO). Results Ten alleles at HLA-A locus, and 19 alleles at HLA-B locus in Luoba ethnic group were detected. Of the 10 HLA-A alleles detected, the three most common alleles were HLA-A*11(allele frequency: 36.40%),-A*02 (25.50%),-A*24 (23.90%), and they covered 85.80% of the total HLA-A alleles detected in the Luoba ethnic group. Of the 19 HLA-B alleles detected, the three most common alleles were HLA-B*40(27.20%), -B*15(11.40%)and -B*38(10 90%), and they covered 49.50% of the total -B alleles detected in the Luoba ethnic group. Conclusion The distribution of HLA-A,-B allele polymorphism in the Luoba nationality is distinctive, but some of the gene distribution in the Luoba group is nearer to that in the Tibetan group. These are consistent with the results of ethnological, historical and sociological researches.

关 键 词：HLA 珞巴族 聚合酶链反应-序列特异性寡核苷酸探针 多态性研究 B基因 反向斑点杂交技术 等位基因多态性 基因的多态性 西藏林芝地区 基因频率分布 无血缘关系 西藏地区 健康个体 基因分型 位点 检出 基因为 人群体 独特性 

分 类 号：Q987[生物学—遗传学]