基于超声波下淀粉纳米颗粒作载体的基因转导  

Starch Nanoparticle as Transgenic Vehicle Mediated by Ultrasound

作　　者：刘俊[1] 刘选明[1] 肖苏尧[1] 童春意[1] 唐冬英[1] 赵李剑[1] 

机构地区：[1]湖南大学生命科学与技术研究院,化学生物传感与计量学国家重点实验室,长沙410082

出　　处：《高等学校化学学报》2005年第4期634-637,共4页Chemical Journal of Chinese Universities

基　　金：湖南省科技厅生物技术重大专项基金(批准号:03NKY1001);教育部重点科技项目基金(批准号:2002-78)资助.

摘　　要：报道了多聚赖氨酸淀粉纳米颗粒(PLL-StNP)在超声波介导下作基因载体的研究,实验发现DNA-PLL-StNPs复合物经过超声波处理不同的时间后的电泳分析显示,结合的DNA受到保护,其生物学性质没有改变;将pIRGFP质粒DNA-PLL-StNPs复合物与COS-7细胞混合,在120W和40kHz的超声波强度下处理2min,细胞表达绿色荧光蛋白的比率最大,达到70%.这种基于超声波下淀粉纳米颗粒作载体的基因转导方法可被广泛应用于动物转基因技术和人类基因治疗,同样可被广泛应用于植物转基因技术.A method in which poly-L-lysine-starch nanoparticle(PLL-StNP) could act as gene vehicle mediated by ultrasound was described in this paper. It was found that when pIRGFP plasmid DNA-PLL-StNP complexes were treated with ultrasound for different time, electrophoresis analysis showed that the biological characteristics of DNA could be well-kept, when DNA-PLL-StNP complexes were transferred into COS-7 cells mediated by ultrasound for 2 min at 120 W and 40 kHz, the cells were expressed GFP at a frequency of about 70%. This method may(will) provide a wide application not only in animal transgenic technique and in human gene therapy, but also in plant transgenic technique.

关 键 词：纳米颗粒 基因载体 超声波 基因转导 

分 类 号：O629.73[理学—有机化学]