分子印迹-化学发光分析法测定安乃近  

Molecular Imprinting-{chemiluminescence} Determination of Analgin

作　　者：何云华[1] 吕九如[1] 张红鸽[1] 杜建修[1] 

机构地区：[1]陕西师范大学化学与材料科学学院,西安710062

出　　处：《高等学校化学学报》2005年第4期642-646,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20275023);陕西省自然科学基金(批准号:2002B12)资助.

摘　　要：以甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,合成了安乃近分子印迹聚合物,对其性能进行了Scatchard分析;以此聚合物为分子识别物质,利用锰()-甲醛-安乃近化学发光体系,结合流动分析技术,建立了高选择性测定安乃近的分子印迹-化学发光分析方法.该方法的线性范围为1.0×10-7～1.0×10-5mol/L,检出限为4×10-8mol/L,对1.0×10-6mol/L安乃近溶液平行测定7次的相对标准偏差为2.4%.该方法已用于尿液中安乃近的直接测定.Chemiluminescence(CL) reaction of manganese(Ⅳ)-formaldehyde-analgin was carried out. Molecular imprinted polymer of analgin was prepared by using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker. The binding characteristic of the analgin-imprinted polymer was evaluated by Scatchard analysis. A new chemiluminescence method for the determination of analgin was proposed by the combination of analgin-imprinted polymer and manganese(Ⅳ)-formaldehyde-analgin CL system. The linear range is 1.0×10^-7 -1.0×10^-5 mol/L, the detection limit is 4×10^-8 mol/L, the relative standard deviation is 2.4%(1.0×10^-6 mol/L analgin, ~n=7 ). The method was applied to the determination of analgin in human urine samples directly.

关 键 词：化学发光 分子印迹 安乃近 锰(Ⅳ) 

分 类 号：O657.39[理学—分析化学]