尿毒症中分子毒物的分离鉴定  

Separation and Identification of the Uremic Middle Molecules

作　　者：杨眉[1] 张建达[1] 王芃[1] 刘晓航[1] 袁直[1] 刘宏富[2] 杨洪涛[3] 杨淑芬[2] 赵菁丽 

机构地区：[1]吸附分离功能高分子材料国家重点实验室,南开大学高分子化学研究所,天津300071 [2]天津市天和医院,天津300050 [3]天津市中医学院第一附属医院,天津300193

出　　处：《高等学校化学学报》2005年第4期677-679,共3页Chemical Journal of Chinese Universities

基　　金：国家"九七三"计划(批准号:G1999064707)资助.

摘　　要：Sera and urine from patients with severe uremia and healthy persons were separated by means of gel permeation chromatography on Sephadex G-15 column with N（C2H5）3-H2CO3 buffer as the eluent. Two middle molecular peaks（A and B） were detected at 206 nm in normal urine, uremic serum and uremic urine, but these two peaks were hardly observed in the profile of normal sera. In contrast, the absorption at 206 nm of fractions A and B from uremic urine were smaller than that of fractions A and B from normal urine. Fractions A from normal urine, uremic serum and urine were collected and resolved into 3 subpeaks at 254 nm by high performance liquid chromatography. Two of these subpeaks, A-Ⅰ and A-Ⅱ, were detected in uremic serum, normal urine and uremic urine. The results of MALDI-TOF-MS revealed that the fraction A-Ⅰ from both uremic serum and normal urine contained a component with molecular weight {1 214}, which could hardly be seen in the fraction A-Ⅰ of uremic urine.Sera and urine from patients with severe uremia and healthy persons were separated by means of gel permeation chromatography on Sephadex G-15 column with N(C_2H_5)_3-H_2CO_3 buffer as the eluent. Two middle molecular peaks(A and B) were detected at 206 nm in normal urine, uremic serum and uremic urine, but these two peaks were hardly observed in the profile of normal sera. In contrast, the absorption at 206 nm of fractions A and B from uremic urine were smaller than that of fractions A and B from normal urine. Fractions A from normal urine, uremic serum and urine were collected and resolved into 3 subpeaks at 254 nm by high performance liquid chromatography. Two of these subpeaks, A-Ⅰ and A-Ⅱ, were detected in uremic serum, normal urine and uremic urine. The results of MALDI-TOF-MS revealed that the fraction A-Ⅰ from both uremic serum and normal urine contained a component with molecular weight {1 214}, which could hardly be seen in the fraction A-Ⅰ of uremic urine.

关 键 词：尿毒症 中分子 高效液相色谱 飞行时间质谱 

分 类 号：Q592.1[生物学—生物化学] Q592.2