糖尿病大鼠海马突触超微结构观察  

The ultrastructure study of the hippocampus' synapses in diabetic rats

作　　者：张松筠[1] 杨晓光[2] 张若青[3] 刘文淑[1] 杨天祝[4] 

机构地区：[1]河北医科大学第二医院内分泌科 [2]河北医科大学第二医院药剂科 [3]石家庄市第一医院,河北石家庄050000 [4]河北医科大学神经生物研究室,河北石家庄050000

出　　处：《基础医学与临床》2005年第3期253-256,共4页Basic and Clinical Medicine

摘　　要：目的观察实验性糖尿病大鼠海马突触超微结构改变及胰岛素治疗的作用。方法将成年SD大鼠随机分为正常对照组、糖尿病组、糖尿病治疗组,应用透射电镜和形态计量学方法观察分析成模第3月末及第6月末海马突触超微结构及结构参数的变化。结果糖尿病组可见突触变性,突触密度、突触后致密物厚度显著减低,突触间隙宽度显著增宽。6月组较3月组更加严重。治疗组仅6月时有显著改变,但较糖尿病6月组明显轻微。结论糖尿病可导致大鼠海马突触数量减少、形态结构发生显著变化,早期应用胰岛素治疗可预防、延缓病变。Objective To study the ultrastructural changes in hippocampus' synapses of diabetic rats and the effect of insulin on these changes. Methods Adult Sprague-Dawley rats(BW 250g±) were divided into normal control group, diabetic group and treatment group. Rats were killed at 3 months and 6 months after Morris water maze test.The Ultrastructural changes of synapses were observed in electron microscope. The number density,the width of synaptic cleft and the thickness of postsynaptic density were studied. Compared to the normal control group,synapses of diabetic group had retrograde changes. Results The number of density, the thickness of postsynaptic density were decreased and the width of synaptic cleft were significantly increased at 3 months(P<0.01), and more significantly at 6 months(P<0.01). In treated group only 6 months groups had such changes. Conclusion There are significant changes in hippocampus' synapses of diabetic rats and early use of insulin could delay the occurrence of neuropathological changes.

关 键 词：海马突触 超微结构观 实验性糖尿病大鼠 胰岛素治疗 超微结构改变 成年SD大鼠 突触后致密物 正常对照组 形态计量学 结构参数 透射电镜 间隙宽度 形态结构 治疗组 触变性 

分 类 号：R587.1[医药卫生—内分泌]