人胃黏膜组织蛋白质组二维电泳图像的获得  

Establishment of a two-dimensional gel electrophoresis map of human gastric mucosa proteome

作　　者：李新华[1] 张万岱[2] 王波涛[2] 肖冰[2] 张振书[2] 

机构地区：[1]青岛大学医学院附属医院消化科,山东青岛266003 [2]第一军医大学南方医院消化科,广东广州510515

出　　处：《基础医学与临床》2005年第3期272-276,共5页Basic and Clinical Medicine

摘　　要：目的初步建立并优化人类胃黏膜组织蛋白质组分析所需的双向凝胶电泳技术,提高其分辨率及重复性。方法刮取手术胃黏膜组织,对以固相pH梯度为第一向的双向凝胶电泳的关键因素与环节,如样品处理、上样量、电泳参数、凝胶浓度和SDS凝胶电泳染色方法等进行一系列的优化。以固相pH梯度———IPG胶条(pH=3～10)进行第一向等电聚焦,以SDS均一胶(13%)的垂直电泳为第二向。结果成功地得到了胃黏膜组织的双向凝胶电泳图谱。Objective Two-dimensional gel electrophoresis(2-DE) is a key technique for proteomics.To analyze the proteome of gastric mucosa and its change in different disease. 2-DE technique is established. Methods A series of important factors, such as sample preparation, electrophoresis parameters, choice of IPG gels, concentration of SDS gels, protocol for staining were optimized to improve the resolution and reproducibility. Results Using the proper method described above, choosing appropriate sample amount, using pre-cast IPG dry strip and casting 13% equal gel, satisfactory 2-DE maps were obtained.

关 键 词：组织蛋白质组 电泳图像 双向凝胶电泳技术 固相PH梯度 胃黏膜组织 二维 蛋白质组分析 凝胶电泳图谱 样品处理 染色方法 等电聚焦 SDS 重复性 分辨率 上样量 IPG 优化 

分 类 号：Q51[生物学—生物化学]