东方百合花器离体培养和快速繁殖研究  

Study on floral organ culture and rapid propagation of oriental hybrid lily

作　　者：柯昉[1] 陈华[1] 黄宇翔[1] 李章汀[1] 

机构地区：[1]福建省农业科学院地热农业利用研究所,福建福州350003

出　　处：《仲恺农业技术学院学报》2005年第1期14-17,共4页Journal of Zhongkai Agrotechnical College

摘　　要：以东方百合(Liliumtenuifoliumorientalhybrid)索邦品种的花瓣、花丝、花托为外植体,就不同激素组合对诱导愈伤组织和不定芽的影响进行了研究.结果表明,索邦百合花瓣、花丝、花托形成愈伤组织的能力有差异,其能力大小依次为花托>花丝>花瓣;愈伤组织诱导的最佳培养基为MS+2,4-D1 0mg/L+BA0 5mg/L,芽最佳分化培养基为MS+BA1 0mg/L+NAA0 1mg/L,芽最佳增殖培养基为MS+KT0 5mg/L+NAA0 1mg/L,生根最佳培养基为1/2MS+NAA0 2mg/L.The floral organ culture of Oriental Lily Sorbonne was studied by using its petals,filaments and receptacles as explants respectively. The best explant was receptacle. The best medium for callus formation was MS+2,4-D 1.0 mg/L+BA 0.5 mg/L. The best medium for bud initiation was MS+BA1.0 mg/L+NAA0.1 mg/L. The best medium for the propagation of cluster shoots was MS+KT0.5 mg/L+NAA0.1 mg/L. The best medium for rooting of seedlings was 1/2MS +NAA 0.2 mg/L.

关 键 词：东方百合(Lilium teruuifoliun ORIENTAL hybrid) 花器培养 快速繁殖 

分 类 号：S682.36[农业科学—观赏园艺]