抗SARS冠状病毒表面抗原抗体的制备及其初步应用  

Preparation and Application of SARS-associated Coronavirus Spike Protein Antibodies

作　　者：许文革[1] 刘一兵[1] 韩世泉[1] 胡轶丁[1] 罗志福[1] 

机构地区：[1]中国原子能科学研究院同位素研究所,北京102413

出　　处：《原子能科学技术》2005年第2期101-105,共5页Atomic Energy Science and Technology

基　　金：中国原子能科学研究院院长基金资助项目(zJ200315)

摘　　要：研究探讨了制备和鉴定SARS 冠状病毒表面抗原的快速免疫检测方法。采用基因工程表达SARS冠状病毒表面抗原免疫BALB/c小鼠,通过杂交瘤技术,获得了4 株稳定分泌高特异性抗SARS冠状病毒表面抗原的杂交瘤细胞系,制取了含高效价单克隆抗体的腹水;采用SARS冠状病毒表面抗原免疫家兔、绵羊、山羊,制取了抗SARS冠状病毒表面抗原的多克隆抗体。研究结果表明:CS4 C11单抗作为示踪抗体、绵羊抗SARS冠状病毒表面抗原抗体作为固相抗体为最佳配对;CS4 C11 单抗作为示踪抗体、CS6B12单抗作为固相抗体次之。获得的抗SARS冠状病毒表面抗原的抗体为SARS的早期诊断研究奠定了基础。Four hybridoma cell lines secreting monoclonal antibody against SARS-associated coronavirus spike protein were obtained and at the same time the polyclonal antibodies were also got by immunizing sheep, goats and rabbits with SARS-associated coronavirus spike protein respectively. Immunoradiometric assay (IRMA) and enzyme-linked immunosorbent assay (ELISA) were established using above Abs for detecting SARS-associated coronavirus, and best assay results were observed when McAbCS4C11 was used as labeled Ab and PcAb(sheep) as capture Ab.

关 键 词：表面抗原 抗SA SARS冠状病毒 抗体 单抗 免疫 腹水 绵羊 山羊 家兔 

分 类 号：R593[医药卫生—内科学] R563.1[医药卫生—临床医学]