药用植物大戟悬浮细胞的培养  

Suspending Cell Culture of Medicinal Plant Euphorbia pekinensis

作　　者：戴传超[1,2] 余伯阳[3] 薛菲[4] 蒋继宏[1] 

机构地区：[1]徐州师范大学江苏省药用植物生物技术重点实验室 [2]南京师范大学江苏省资源生物技术重点实验室,江苏南京210097 [3]中国药科大学中药学院 [4]南京师范大学江苏省资源生物技术重点实验室

出　　处：《南京林业大学学报（自然科学版）》2005年第2期57-60,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：江苏省药用植物生物技术重点实验室开放项目资助(KJS03079)

摘　　要：比较了激素促进大戟叶、茎及腋芽形成的愈伤组织,得出 MS培养基+2.0 mg/L 6 BA+0.4 mg/LNAA最有利于形成愈伤,腋芽是获得愈伤的最佳材料。经过多代转接,获得大戟悬浮细胞系,培养21天生物量达到最大,为10.82 g/L。加入大戟内生菌(镰刀菌)的菌丝提取液,可以获得比对照高 34.13%的细胞产量;比较组织培养细胞HPLC指纹成分表明,悬浮培养细胞含有野生大戟药材的主要成分;加入内生菌提取物,其指纹组分含量及产量发生改变。The phytohormone composition to produce callus by the leaf,stem,gemmules of Euphorbia pekinensis were compared.It was the best phytohormone composition that adding 6-BA((2.0mg/L)),NAA 0.4mg/L to MS culture medium.Gemmule was the best explant to produce callus.By trans-cultured for many times,the suspending cell could be get.The biomass got highest yield at 21d.It got 10.82g/L.The biomass could get 34.13% increased if adding the endophytic fungi (one kind of Fusariium sp.) mycelium’s extract.The compositions of suspending cells enthol extract were compared with that of wild.There was the same in the main composition.The concentration and yield of main composition changed when the mycelium extract to the culture medium were added.

关 键 词：大戟 悬浮细胞 愈伤组织 内生菌诱导子 活性成分 

分 类 号：Q813[生物学—生物工程]