氟烷和安氟醚对大鼠心肌缺血再灌注损伤的保护作用  

Protective effects of halothane and anflurane on myocardiac ischemia-reperfusion injury

作　　者：李恒[1] 杨承祥[1] 李卫东[2] 董晓莉[2] 孟宪慧[2] 曾因明[3] 王钧[3] 

机构地区：[1]广东佛山市第一人民医院麻醉科,广东佛山528000 [2]河南省胸科医院麻醉科,河南郑州450001 [3]徐州医学院江苏省麻醉学重点实验室,江苏徐州221000

出　　处：《中国现代医学杂志》2005年第6期847-850,857,共5页China Journal of Modern Medicine

基　　金：河南省科委科技攻关计划项目;No:001170624

摘　　要：目的研究氟烷和安氟醚对缺血再灌注心肌功能和代谢、氧自由基的影响。方法SD大鼠80只,随机分为10小组,每组8只。采用Langendorff离体大鼠心脏模型。按给药方式又分为3大组,对照组(含4小组):平衡15min为1小组,平衡后续灌15min为1小组,平衡续灌后缺血10min为1小组,平衡续灌缺血25min后复灌30min为1小组。氟烷组(含3小组):平衡15min后,灌注含1.5MAC氟烷灌注液15min为1小组,平衡续灌后缺血10min为1小组,平衡续灌缺血25min复灌含1.5MAC氟烷的灌注液30min为1小组。安氟醚组:包括3小组,情况同氟烷组。各组记录平衡后,给药后(或续灌15min)复灌30min左室收缩压(LVSP)、左室舒张末期压(LVEDP)、左室发展压(LVDP)、左室压力升高或降低最大速率(±dp/dtmax)、心率(HR)、冠脉流量(CF)。实验结束后测定心肌超氧化物歧化酶(SOD)活性、心肌丙二醛(MDA)含量、高能磷酸盐(ATP)含量。结果安氟醚具有明显扩张冠状动脉的作用。安氟醚能促进缺血再灌注心肌冠脉流量的恢复。两用药组明显降低LVDP、+dp/dt,升高LVEDP(P<0.05);缺血再灌注后,氟烷、安氟醚的LVDP分别恢复到基础值的57%、62%,+dp/dt分别恢复到基础值的56%、67%;与对照组相比差异具有显著性。两用药组均能提高心肌ATP含量,缺血后心肌ATP下降较慢,复灌后恢复较快。To investigate the effect of Halothane and Enflurane on function and metabolism and free radicals in isolated ischemia /reperfusion(I/R) rat hearts. 80 SD rats were anesthetized with 3% pentobarbital 30 mg.kg -1,it were randomly divided into 10 little groups (n =8), or 3 big groups according to given drug. In a normal thermal isolated Langendorff rat heart model, two volatile anesthetics in 1.5 MAC concentration were given before global ischemia and after reperfusion. Three big groups were studied. Group Ⅰ (contain 4 little groups, control group) received 30 min of perfusion and then 25 min of ischemia followed 30 min of reperfusion. All the treatment groups(group Ⅱ: 1.5 MAC halothane;group Ⅲ: 1.5 MAC enflurane) received 15 min of perfusion and 15 min of drug treatment followed by 25 min of ischemia and 30 min of reperfusion. Every big group contain3 little groups. A latex balloon was placed in the left ventricle to obtain isovolumetric contraction. Coronary flow (CF),left ventricular diastolic pressure (LVEDP),left ventricular developed pressure (LVDP),+dp/dt and -dp/dt were monitored at 15 min of equilibrium, 15 min of drug treatment, the end of reperfusion. Myocardial Adenosine triphosphate (ATP), Malodialdehyde(MDA), Activity of Superoxide Dismutase (SOD)were determined at 15 min of equilibrium , 15 min of drug treatment or absence, 10 min global ischemia and the end of reperfusion. 15 min treatment with volatile anesthetics resulted in a significant decrease in LVDP, + dp/dt. CF was increased significantly after exposure to enflurane, but not halothane. Also the myocardial ATP content were significantly increased after exposure to anesthetics. After 10 min ischemia the myocardial ATP content decreased slowly in treated groups. At the end of reperfusion, ventricular function, CF, myocardial ATP content and the activity of SOD in the treated groups were significantly higher than those in control group. Myocardial MDA content in treated groups was significantly lower than those in the control gr

关 键 词：氟烷 安氟醚 心肌再灌注损伤 丙二醛 SOD ATP 

分 类 号：R965[医药卫生—药理学]