黄芪多糖对小鼠腹腔巨噬细胞免疫功能的调节作用研究  

Study on immunomodulatory effect of Astragalus Polysaccharide on mice peritoneal macrophage

作　　者：姚金凤[1] 王志新[2] 张晓勇 张瑞峰[4] 

机构地区：[1]郑州大学药学院,河南郑州450052 [2]河南大学医学院,河南开封475001 [3]南阳医专附属医院,河南南阳473000 [4]郑州大学第一附属医院,河南郑州450052

出　　处：《河南大学学报（医学版）》2005年第1期34-36,共3页Journal of Henan University:Medical Science

摘　　要：目的:研究黄芪多糖(APS)增强巨噬细胞的免疫作用,探讨其调节机体免疫功能的机理。方法:在体外培养的小鼠腹腔巨噬细胞中加入不同浓度的APS后,观察其对巨噬细胞一氧化氮(NO)合成及对肿瘤细胞杀伤活性的影响。结果:APS能促进巨噬细胞NO合成,并能显著增强小鼠腹腔巨噬细胞对黑色素瘤细胞的杀伤作用。结论:APS能增强小鼠腹腔巨噬细胞的免疫作用,可能是其抗肿瘤作用的机理之一。Objective: To investigate the immunomodulatory effect of Astragalus Polysaccharide on macrophage and explore the mechanism of its immunomodulatory.Methods: By adding different concentration of APS into cultured mice peritoneal macrophage in vitro, the influence of APS on the synthesis of nitro oxide (NO) was observed. The microculture tetrazoli-um (MTT) assay was used to determine the cytotoxicity of macrophage induced by APS.Results: APS could significantly increase NO synthesis and enhance the effects of mice peritoneal macrophage on killing carcinoma cells. Conclusion: APS can enhance the immune function of macrophage, which is one of the mechanism of anticancer.

关 键 词：黄芪多糖 巨噬细胞 免疫调节 黑色素瘤细胞 

分 类 号：R979.1[医药卫生—药品]