利用rDNA的PCR-RFLP对伞滑刃属线虫群体的分子鉴别  

Molecular identification of some Bursaphelenchus populations with the aid of PCR-RFLP of rDNA

作　　者：蒋立琴[1] 梁定东[1] 郑经武[1] 顾建锋[2] 杨兰英[2] 

机构地区：[1]浙江大学植物保护系,浙江杭州310029 [2]宁波出入境检验检疫局检验检疫中心,浙江宁波315012

出　　处：《浙江大学学报（农业与生命科学版）》2005年第2期161-164,共4页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江省科技厅项目(2002C34003);浙江省自然科学基金资助项目(300267).

摘　　要：应用对rDNA中ITS的PCR-RFLPs技术和方法对来自中国、日本和韩国的松树或木质包装箱中分离出来的伞滑刃属(Bursaphelenchus)6个线虫群体进行了分子鉴定.其中来自浙江镇海的松树和日本的木质包装箱中分离出的各一线虫群体被鉴定为松材线虫(B.xylophilus),而来自浙江富阳的松树和韩国、香港及日本的木质包装箱中的另一个群体等4群体被鉴定为拟松材线虫(B.mucronatus).本研究中选用的4种限制性内切酶(Hinf 、Hae 、Msp 、cfo )的特异性酶切位点均可以有效地鉴别松材线虫和拟松材线虫.Six populations of genus Bursaphelenchus isolated from pine wood produced in China, Japan and Korea were identified with the aid of PCR-RFLP of rDNA. It was bound that one of the populations, which had its origin in the forests of Zhenhai, Zhejiang Province, China, and the other, which was derived from imported coniferous wood packages produced in Japan were both identified as B. xylophilus. The other four populations, including populations from Fuyang of the Zhejiang Province, Korea, Hongkong and Japan, were identified as B.mucronatus. All the four restriction enzymes, namely HinfⅠ, HaeⅢ, MspⅠ, and CfoⅠ, with their special sites of cutting could be used to differentiate the two morphologically very similar species.

关 键 词：松材线虫 拟松材线虫 核糖体DNA 分子鉴别 

分 类 号：S41[农业科学—植物保护]