家猪rob（13；17）的联会复合体分析  

Synaptonemal Complex Analysis of 13/17 Robertsonian Translocation in the Domestic Pig

作　　者：单继东[1] 张传善[1] 孙金海[1] 李来记[1] 

机构地区：[1]东北师范大学生物系,中国人民解放军兽医大学畜牧系

出　　处：《Acta Genetica Sinica》1994年第2期96-103,共8页

摘　　要：经对亲代公猪以及其部分子代公猪的染色体进行Ｇ－带和Ｃ－带分析，发现它们均携有ｒｏｂ（１３；１７），其染色体组成为２ｎ＝３７，ＸＹ，ｒｏｂ（１３；１７）．本文采用表面铺展法，硝酸银染色技术对这些杂合易位公猪的ｒｏｂ（１３；１７）减数分裂的配对行为进行了联会复合体（Ｓｙｎａ－ｐｔｏｎｅｍａｌＣｏｍｐｌｅｘ，ＳＣ）的电镜观察和分析，结果表明：ｒｏｂ（１３；１７）的（未配对的）ＳＣ侧生组分与１３，１７二（未配对的）ＳＣ侧生组分配对形成一个三价体，三价体均呈顺式构型（ｃｉｓｃｏｎｆｉｇｕｒａｔｉｏｎ）且有延迟联会现象。我们对不同时期的三价体（三价体的联会发育顺序是根据ＸＹ二价体联会行为而确定的）的各个部位进行了测量，发现ｒｏｂ（１３；１７）的形成伴随着染色体缺失，缺失部位在１３，１７近端部。联会过程中，还发现三价体配对有长度调节（ｌｅｎｇｔｈａｄｊｕｓｔｍｅｎｔ）现象：Ⅰ．１３，１７二近端部ＳＣ侧生组分形成非同源联会，成为三价体中的短ＳＣ侧臂，而且短ＳＣ侧臂的长度变化不定。Ⅱ．三价体中短ＳＣ侧臂在长ＳＣ臂ｒｏｂ（１３；１７）与１３、１７（同源配对形成的）上的位置发生移动，由原来的位于长ＳＣ的１／４处移动到了１／１０处?G and C-banding study of the chromosomes of several male pigs,indicate that all of these pigs carry rob(13;17).The karyotype is 2n=37,XY,rob(13;17).In this paper,silverstained Synaptonemal Complexes(SCs)in surface-spread pachytene nuclei from these pigs are analysed by electron microscope. The result is as follows:Robertsonian translocation metacentrics rob(13;17) paired with homologous acrocentrics(13,17),forming a trivalent.All trivalents are in a cis configuration and there is delayed pairing in the trivalent.We measured every part of trivalent in the different stage of prophase(based on the morphology of XY-bivalent).It is shown that accompanying with rob(13;17) formation there has been a deletion in the proximal part of 13,17 translocation chromosome.In the process of synapsis,the length adjustment phenomenon in the trivalent occured:a. There is nonhomologous synapsis between the two short arms of the acrocentrics(13;17).The length of short SC varied.b.The position of the short SC,which is on the long SC(homologous synapsis of rob(13;17)and 13,17) moved,from one-fourth of long SC to one-tenth of it, near the end of 17 arm. These phenomenon is the result of a gradual length adjustment of lateral elements of 13,17 to the lateral element of the rob(13;17).We has discussed the mechanism of the trivalent synaptic adjustment,and it’s important use to control the chiasmata and disjunction of the chromosomes.

关 键 词：家猪 三价体 联会复合体 染色体 

分 类 号：S828.2[农业科学—畜牧学]