HeLa细胞的反式作用因子CTF／NF－1与其顺式作用元件的特异性作用  

The Specific Reaction Between the Trans-Acting Factor,CTF/NF-1 and the Related Cis-Acting Element

作　　者：谷春[1] 刘连瑞[1] 王恢鹏[1] 冯尚[1] 杨涛兰[1] 

机构地区：[1]中国科学院遗传研究所

出　　处：《Acta Genetica Sinica》1994年第4期321-329,共9页

基　　金：中国科学院遗传研究所所长基金

摘　　要：本文运用凝胶延迟反应（ＧｅｌＲｅｔａｒｄａｔｉｏｎＡｓｓａｙ）和足迹法（Ｆｏｏｔｐｒｉｎｔｉｎｇ）研究了ＨｅＬａ细胞核蛋白中的反式作用因子ＣＴＦ／ＮＦ－１与其对应的顺式作用元件的特异性作用。研究结果表明ＨｅＬａ细胞的ＣＴＦ／ＮＦ－１包含有不同分子量的组分，它们均能特异性结合于特异位点ＡＴＡＴＴＧＧＣＴＴＣＡＡＧＣＣＡＡＡＡＴＧＧＡ序列，形成至少５种不同分子量形式的因子－元件复合物。这一结果对阐明ＣＴＦ／ＮＦ－１复杂多样的调控机制及其组成种类有重要意义。此外，本文还建立了一种制备核蛋白提取物的新方法，有效地解决了因反式作用因子含量极低带来的研究上的困难。The specific reaction between the trans-acting factor,CTF/NF-1, and the related cis-acting element was studied. On gel retardation assay and footprinting analysis. it was demonstrated that there were at least five composed elements of CTF/NF-1 in the nuclear extracts of HeLa cells. All of them can specifically bind to 0the DNA sequence ATATTGGCTTCAAGCCAAAATGGA in vitro, As a result. at least five specific compounds of the protein-nuclear acid were formed. These results can help us to learn the composition of CTF/NF-1 and explain the complicated functional mechanism of CTF/NF-1 in the process of DNA replication and transcription. In this paper, we also built a new method used for preparing the nuclear extracts. Consequently, the problem was resolved which is caused by very slight quantity of the factors contained in eukaryotic cells.

关 键 词：反式作用因子 顺式作用 HELA细胞 

分 类 号：Q343.1[生物学—遗传学]