盐生盐杆菌的质粒及其物理图谱  

Plasmid from Halobacterium halobium and Its Restriction Map

作　　者：沈萍[1] 陈娅静 

机构地区：[1]武汉大学生命科学学院

出　　处：《Acta Genetica Sinica》1994年第5期409-416,共8页

基　　金：国家自然科学基金

摘　　要：本文用４种不同的方法对１０株盐生盐杆菌（Ｈａｌｏｂａｃｔｅｒｉｕｍｈａｌｏｂｉｕｍ）是否含有质粒进行了检测，其中６株（Ｓ９、ＲＰ１、Ｊ７、Ｒ６－４、Ｒ６－５、Ｔ４－１）含有质粒，均属大质粒。几种检测方法中，以ＴＥＮＳ法效果最好。Ｊ７菌株含有１种ＣＣＣ结构十分稳定的质粒，称之为ｐＨＨ２０５，其分子量较小，约为１６．７ｋｂ；拷贝数较高，为４９个／每个细胞。该质粒在宿主的对数生长后期出现拷贝数高峰。通过限制性酶切分析，确定了５种酶在ｐＨＨ２０５上的切点，其中ＢａｍＨＩ、ＨｉｎｄＩＩＩ和ＸｂａＩ３种酶为单一切点，而且彼此十分邻近。In this paper,ten strains of H.halobium were screened for the ocurrence of plasmid by four methods.Six of the strains harbored plasmids of high molecular weight,Of all the detecting methods,the TENS method was the best for H.halobium, J7 strain containes only one type of plasmid named as pHH205 which showep stationary CCC form. The highest copy number of the plasmid was observed in late stationary growth phase, which was appromately 49 copies per cell. The molecular weight is 16 .7kb. The sites of five endonucleases had been determined for pHH205 and three of them, namly BamHI, HindIII and XbaI, were single sites neighboring to each other.

关 键 词：盐生盐杆菌 质粒 物理图谱 

分 类 号：Q939.110.3[生物学—微生物学]