普通小麦与鸭茅状摩擦禾的远缘杂交──Ⅱ．未成熟胚的培养  

Wild Hybrization Between Triticum aeativum and Tripsacum dactyloides II. Immature Embryo Cultured

作　　者：李大玮[1] 欧阳平[1] 姚庆筱[1] 邱纪文[1] 

机构地区：[1]中国科学院遗传研究所植物细胞与染色体工程国家重点实验室

出　　处：《Acta Genetica Sinica》1994年第6期474-478,共5页

基　　金：中国科学院重大科研项目

摘　　要：培养了由２６个小麦（Ｔｒｉｔｉｃｕｍａｅｓｔｉｖｕｍ，２ｎ＝６ｘ＝４２）品种（系）用鸭茅状摩擦禾（Ｔｒｉｐｓａｃｕｍｄａｃｔｙｌｏｉｄｅｓ，２ｎ＝４ｘ＝７２）的花粉授粉１４天后获得的６４５个未成熟的胚。结果表明，未成熟胚培养的植株再生频率与胚来源的小麦基因型的杂交亲和性无关。胚的发育程度直接影响胚培养的结果。离体时分化完全的未成熟胚在无激素的培养基上可以迅速萌发成苗，而分化未完全的小胚在无激素的培养基上分化进程不能继续，而且在无激素补充的情况下，萌发过程一旦起动，即使将这些胚转至补加了激素的胚分化培养基上，分化过程也不能再补救。讨论了提高未成熟胚培养成苗率的关键和措施。The total 645 immature embruos (after 14 days pollination) from 26 wheat (Triticum aestivum, 2n=6x=42) varieties(lines)crossed with Tripsacum dactyloides (2n=4x=72) were cultured on a hormone-free medium. The results indicated that there were no immediat relations between the frequencies of plant regeneration of cultured embryos and wheat genotypes from which the embryos originated. Well-differentiated immature embryos could speedily germinated and developed into plantlets on the hormone-free medium, while differentiation process of incomplete-differentiated small embryos could not be continued on the same medium. They grew only codeopeiloor, coleopcilc and radicies, and even failed to germinate. Afterwards those cultures with coleopcile or radicles and coleopcilc were transferred on an embryo differentiation medium containing 0.5mg/L KT and 0.5mg/L IAA,their differentiation processes were past remedy. The problems about immature embryo development and regeneration were also discussed.This subject supported by key research subjects of The Chinese Academy of Sciences.

关 键 词：小麦 鸭茅状摩擦禾 远缘杂交 未成熟胚培养 

分 类 号：S512.103.5[农业科学—作物学]