具有谷胱甘肽过氧化物酶活性的催化抗体的研究Ⅰ．谷胱甘肽特征全抗原的合成、表征及性质  

Studies on Catalytic Antibody with Glutathione Peroxidase Activity Ⅰ. Synthesis and Property of Glutathione Derivatives as Specific Antigens

作　　者：朱振齐[1] 丁兰[1] 罗贵民[1] 孙启安 杨同书[1] 沈家骢[1] 

机构地区：[1]吉林大学酶工程国家重点实验室,吉林大学分子光谱和分子结构开放实验室

出　　处：《应用化学》1994年第5期87-90,共4页Chinese Journal of Applied Chemistry

摘　　要：用２，４－二硝基氯苯保护谷胱甘肽的巯基制出半抗原，再通过戊二醛共价反应使其与牛血清白蛋白表面的氨基结合，经超胶ＡｃＡ５４凝胶层析纯化，制备出有较强免疫原性的谷胱甘肽全抗原。用元素分析、红外光谱及核磁共振表征了半抗原的结构。电泳分析得全抗原分子量平均为８７０００道尔顿。光谱分析及圆二色谱研究表明其有较强的可见、紫外及荧光特征吸收，且抗原结构的紧密性增强。The sulfhydryl groups of the reduced glutathione(GSH) have been reacted with2,4-dinitrochlorobenzene to give S-protective glutathione as a hapten, The haptens werecrosslinked with the amino groups on the surface of BSA(Bovine Serum Albumin ) glutaraldehyde,The covalent conjugates are GSH specific antigens of high immunogenicity.The antigens were found to be homogenious and have a molecular weight of 87000 dalton asdetermined by gel-electrophoresis method.Spectral and circular dichroism measurementsshowed that the antigens possess special vis-UV spectra , fluorescence nature with aincreased compact of antigen structure.

关 键 词：谷胱甘肽 过氧化物酶 催化抗体 

分 类 号：Q554[生物学—生物化学]