模拟谷胱甘肽过氧化物酶活性三肽的合成及表征  

Synthesis and Characterizaion of Mimic Tripeptides with GPX Activity

作　　者：朱振齐[1] 孙启安 丁兰[1] 罗贵民[1] 李维[1] 沈家骢[1] 

机构地区：[1]吉林大学酶工程国家重点实验室,吉林大学分子生物学系,吉林大学化学系

出　　处：《应用化学》1994年第6期77-79,共3页Chinese Journal of Applied Chemistry

摘　　要：用Ｂ，Ｂ－二氟硼杂唑烷酮保护谷氨酸的α－氨基和α－羧基，通过液相合成法制得谷氨酰－γ－丝氨酰－甘氨酸三肽。结合苯甲基磺酰氟（ＰＭＳＦ）与硒化氢（Ｈ２Ｓｅ）的突变，再经纯化制得具有谷胱甘肽过氧化物酶活性的三肽模拟物，即γ－谷氨酰－硒代半腕氨酰－甘氨酸。元素分析、氨基酸分析均证明了两种三肽的结构。电子能谱技术分析测得硒的价态及含量。The alpha-amino and alpha-carboxyl group in glutamic acid(Glu)were protectedby specific coprotection method,γ-Glu-Ser-Gly was synthesized by the liquid peptidemethod. The prepared mimic tripeptides were used for the chemical mutation of PMSF andH2Se, as well as γ-Glu-SeCys-Gly, The structures of mimic tripeptides were determined byelemental analysis and amino acid analysis. The valency and quantity of Se in mimic tripep-tides were determined.

关 键 词：三肽模拟物 谷胱甘肽 过氧化物酶 合成 表征 

分 类 号：Q554.6[生物学—生物化学] TQ464.8[化学工程—制药化工]