花楸嫩叶和茎段的愈伤组织诱导  

Callus Induction from Tender Leaf and Stem Segment Explants of So rbus pohuashanensis

作　　者：王爱芝[1] 沈海龙[1] 黄剑[1] 李长海[2] 范少辉[3] 

机构地区：[1]东北林业大学,哈尔滨150040 [2]黑龙江省森林植物园 [3]中国林业科学院林业研究所

出　　处：《东北林业大学学报》2005年第2期12-14,共3页Journal of Northeast Forestry University

基　　金：国家"十五"攻关课题 ( 2001BA510B08 );哈尔滨市科技攻关课题(2003AA6CN094)资助。

摘　　要：以花楸嫩叶和茎段为外植体,在添加BA、TDZ、IBA、NAA、2,4-D组成的不同激素组合的MS培养基上培养,以诱导愈伤组织.以幼嫩的茎段为外植体时,从愈伤组织的诱导率、体积和生长状态综合考虑,得出生长素的效应为NAA＞2,4-D＞IBA;最佳激素种类组合为NAA1.0mg/L+BA0.5～3.0mg/L、2,4-D1.0mg/L+BA0.5～3.0mg/L或TDZ0.01～0.5mg/L.以幼嫩的叶片为外植体时,虽然诱导率可达100%,但只是在切口处长小米粒大小的愈伤组织,生长素的诱导效应大小为2,4-D＞NAA＞IBA,最佳激素组合为2,4-D1.0mg/L+BA0.5～3.0mg/L.将初代培养的愈伤组织转入增殖培养基,增殖效果都非常好,4周后愈伤组织长满培养瓶.Tender leaf and stem segment of Sorbus pohuashanensis were cultured for callus induction on MS medium supplemented with different hormone combinations including BA, TDZ, IBA, NAA and 2,4-D. Considering synthetically from the induct ion rate, volume and growth status of callus, we obtained that the effect sequen ce of auxin was NAA>2,4-D>IBA and the optimum hormone combination was 1.0mg/ L NAA+0.5～3.0mg/L BA, 1.0mg/L 2,4-D+0.5～3.0mg/L BA or 0.01～0.50mg/L TDZ when tender stem segment was taken as explants. Although the induction rate could reach 100%, the callus was very small and induced only from the cut when tender leaf was taken as explants. The effect sequence of auxin was 2,4-D>NAA> IBA and the optimum hormone combination was 1.0mg/L 2,4-D+0.5～3.0mg/L BA. When the callus in first culture was transferred onto proliferation medium, they would become full of bottle after four weeks.

关 键 词：花楸 嫩叶 茎段 愈伤组织 激素组合 

分 类 号：S722.37[农业科学—林木遗传育种]