窒息对新生大鼠肾组织一氧化氮和一氧化氮合酶含量的影响  

Influence of Asphyxia on Nitric Oxide and Nitric Oxide Synthase Levels in Renal of Neonatal Rats

作　　者：于娟 陈正跃[2] 杨玲[3] 邢陆伟[4] 郭宏昌 崔景彬[4] 

机构地区：[1]河南省现代医学研究院医院,郑州450052 [2]新乡医学院,河南新乡453003 [3]郑州大学第二附属医院,郑州450014 [4]郑州大学医学院,郑州450052

出　　处：《实用儿科临床杂志》2005年第4期340-341,共2页Journal of Applied Clinical Pediatrics

基　　金：郑州市科技攻关项目资助(03BA02BMYB05)

摘　　要：目的探讨一氧化氮合酶(NOS)和一氧化氮(NO)在窒息后肾损伤中的作用。方法Wistar新生大鼠48只,随机分为对照组、窒息后复氧2h组、24h组和48h组,制备常压窒息模型。窒息30min后在上述时间点处死动物,测定肾脏NOS和NO含量,并在光镜下对肾小管损伤程度进行评分。结果窒息复氧2h肾脏NOS和NO即显著升高,持续时间达24h,肾小管评分在窒息后24h和48h明显增高。结论NOS和NO在新生大鼠窒息后的肾损伤中起重要作用。Objective To study the effects of nitric oxide synthase(NOS) and nitric oxide(NO) in post-asphyxial renal injury in neonatal rats.Methods Forty-eight Wistar neonatal rats were randomly divided into 4 groups:controls,2 h,24 h and 48 h post-asphyxia groups (12 in each group).The rats were decapitated in different times(2 h,24 h and 48 h) after asphyxia for 30 minutes.The renals were dissected to determined the concentrations of NO and NOS.And the scores of renal tubules were measured under light microscope.Results Compared with control group,the levels of NO and NOS significantly increased at 2 h and 24 h after asphyxia.The scores of renal tubules were significant difference at 24 h and 48 h after asphyxia compared to controls.Conclusion These findings suggest NOS and NO may play an important role in the development of post-asphyxia renal injury.

关 键 词：一氧化氮 一氧化氮合酶 窒息 肾损伤 

分 类 号：R722.12[医药卫生—儿科]