东北鹤虱中一个具有抗菌活性的新喹酮类生物碱  

A new quinolone alkaloid with antibacterial activity from Lappula echinata

作　　者：张韶瑜[1] 孟林[1] 高文远[2] 贾伟[3] 段宏泉[4] 

机构地区：[1]天津医科大学基础医学院,天津300070 [2]天津大学药物科学与技术学院,天津300072 [3]上海交通大学药学院,上海200030 [4]天津医科大学药学院,天津300070

出　　处：《中草药》2005年第4期490-492,共3页Chinese Traditional and Herbal Drugs

基　　金：天津医科大学科研基金资助(03520026)

摘　　要：目的对东北鹤虱的化学成分进行分离和结构鉴定并进行抗菌活性测定。方法通过硅胶柱色谱、凝胶柱色谱等分离方法从东北鹤虱的BuOH提取物中分离得到一个新喹酮类化合物,以有机波谱方法(1H-NMR、13C-NMR、2D-NMR、HR-MS,UV和IR等)进行结构鉴定,并以纸片琼脂扩散法进行抗菌活性测定。结果从东北鹤虱中分离出一个新喹酮类生物碱,鉴定为8-甲氧基-4-喹酮-2-羧酸,并对铜绿假单胞菌、肠致病性大肠埃希菌、肺炎克雷伯菌及表皮葡萄球菌具有抗菌活性。结论该化合物为一新化合物,并具有抗菌活性。Objective To investigate the chemical constituents of Lappula echinata and determine the antibacterial activity.Methods A new quinolone alkaloid was isolated from the BuOH extract of L.echinata by silica gel column chromatography and gel column chromatography.Its structure was identified by 1H-NMR, 13C-NMR, 2D-NMR, HR-MS, UV, and IR spectral data analysis.Its antibacterial activity was determined by KB method.Results A new quinoloe alkaloid named 8-methoxy-4-quinolone-2-caboxylic acid was isolated from L.echinata and was found to have antibacterial activity on Pseudomonas pyocyanea ATCC 27853, EPEC O111, pneumobacillus and Staphylococcus epidermidis.Conclusion This is a new compound with antibacterial activity.

关 键 词：东北鹤虱 核磁共振波谱 喹酮 抗菌活性 

分 类 号：R284.1[医药卫生—中药学]