吴茱萸水溶性成分指纹图谱研究  

Fingerprint study on water soluble component in Evodia rutaecarpa(Juss. )Benth

作　　者：甄攀[1] 张万明[1] 白雪梅[1] 王治宝[1] 王德宝[1] 

机构地区：[1]河北北方学院,河北张家口075000

出　　处：《中国药学杂志》2005年第6期411-414,共4页Chinese Pharmaceutical Journal

摘　　要：目的建立吴茱萸水溶性成分的HPLC指纹图谱,为鉴定吴茱萸药材提供依据。方法色谱柱:YWG-C18(250 mm×4.6 mm,10μm),流动相:乙腈-0.4%磷酸梯度洗脱,流速:0～8 min为1.0 mL·min-1,8-20 min为0.5 mL·min-1,20-35 min为1.0 mL·min-1,检测波长327 nm,柱温30℃,进样量20μL。结果测定的10批吴茱萸样品有15个共有峰,共有峰相对保留时间的RSD为0.24%～1.3%,相对峰面积的RSD为11.8%～59.3%。10批样品之间的重叠率均在0.80以上,共有峰占总峰面积均约在90%。结论本实验首次建立了中药吴茱萸中水溶性成分的HPLC指纹图谱,为吴茱萸药材的真伪鉴别提供了科学依据。OBJECTIVE: To establish a HPLC fingerprint of water soluble component in Evodia rutaecarpa (Juss.) Benth for identifying and evaluating its quality. METHODS: Water extract of Evodia rutaecarpa (Juss.) Benth was separated on a YWG-C18 column (250 mm × 4.6 mm, 10 μm). The mobile phase consisted of acetonitrile-0.4% phosphoric acid with gradient elution. The flow rates were 1.0 mL&middotmin-1 (0-8 min), 0.5 mL&middotmin -1 (8-20 min) and 1.0 mL&middotmin-1 (20-35 min). The wavelength of UV detection was 327 nm. The column temperature was at 30°C and 20 μL was injected. RESULTS: There were 15 common peaks in the fingerprint of 10 samples. The RSDs of the relative retention time of the common peaks was 0.24%-1.3%. The RSD of the relative peak area was 11.8%-59.3%. The overlap rates of the 10 samples were more than 0.80. The percent of the common peaks area in total peaks area were about 90%. CONCLUSION: The fingerprint of water soluble component in Evodia rutaecarpa (Juss.) Benth was firstly established. It will be used to identify and evaluate the quality of Evodia rutaecarpa (Juss.) Benth.

关 键 词：吴茱萸 水溶性成分 指纹图谱 绿原酸 

分 类 号：R284.1[医药卫生—中药学]