不同方法固定的细胞色素C对一氧化氮的响应性能  

The response characteristics to NO with cytochrome C immobilized by different methods

作　　者：代晶[1] 吕太平[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2005年第2期118-121,共4页West China Journal of Pharmaceutical Sciences

摘　　要：目的　比较不同方法固定的细胞色素C(CytC)对一氧化氮(NO)的响应性能。方法　以不同的溶胶凝胶法对CytC进行固定,并通过UV法考察固定化CytC的性质及其对一氧化氮响应的性能。结果　以硅溶胶法固定的CytC膜性质较好,对NO的响应时间较快,敏感度较高,可逆速度快,重复性好,NO在1 2 3～2 85 μmol·L-1 范围内与吸光度线性关系良好(r =0 . 9995 )。结论　为进一步研制NO光学传感器奠定了基础。OBJECTIVE To compare the response characteristics to nitric oxide of Cytochrome C (Cyt C) immobilized by different methods. METHODS Cyt C was encapsulated with several sol-gel procedures. The properties of immobilized Cyt C and their response characteristics to nitric oxide were also studied by UV. RESULTS The colloidal silica-sol method was selected for encapsulation of Cyt C because of its quick response, high sensitivity and good reversibility. NO was linear in the range of 123-285 μmol·L -1 (r=0.9995).CONCLUSION The basement was established for the development of optical nitric oxide sensors.

关 键 词：溶胶凝胶 细胞色素C 一氧化氮 固定 响应性能 

分 类 号：R917[医药卫生—药物分析学]