RP-HPLC测定小鼠口服赤芍吴茱萸复方后血浆中芍药苷的浓度  

Determination of paeoniflorin concentration in mice plasma after oral administration of Chishao Wuzhuyu fufang by RP-HPLC

作　　者：杨祖贻[1] 裴瑾[2] 刘荣敏[1] 程佳[1] 万德光[2] 胡荣[2] 

机构地区：[1]四川省肿瘤研究所,四川成都610041 [2]成都中医药大学药学院,四川成都610075

出　　处：《华西药学杂志》2005年第2期127-129,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家中医药管理局科研基金 (0 2 -0 3ZP5 0 ) ;四川省中医管理局科研基金 (2 0 0 2 17)

摘　　要：目的　建立测定小鼠口服赤芍吴茱萸复方后血浆中芍药苷的方法。方法　采用HPLC法,C1 8柱(2 5 0mm×4 .6mm ,7μm) ,甲醇-水(38∶6 2 )为流动相,流速0 .5ml·min-1 ,检测波长2 30nm。结果　芍药苷与血浆中其他成分能很好分离,芍药苷在5 .0～2 5 0 . 0ng·μl-1 范围内线性关系良好(r=0 .9999) ,RSD <1 0 %,平均回收率95 . 5 2 %,最低检测浓度1 .49ng·μl-1 。结论　本法快速、灵敏、准确,可用于芍药吴茱萸复方在小鼠体内芍药苷的血浆浓度分析。OBJECTIVE To determine paeoniflorin in mice plasma. METHODS An HPLC method was adopted.The conditions of chromatography:A C 18 radial compression column (250 mm×4.6 mm,7 μm) was used with a mobile phase of CH 3OH-H 2O (38∶62). The detectve wavelength was 230 nm and flow rate was 0.5 ml·min -1. External standard method was used for quantitative analysis.RESULTS The linear range was 5.0-250.0 ng·μl -1(r=0.9999). The minimum detection limit was 1.49 ng·μl -1,the average recovery was 95.52%. CONCLUSION This method provides an accurate and sensitive way in detecting plasma concentration of paeoniflorin.

关 键 词：芍药苷 赤芍吴茱萸复方 高效液相色谱 血药浓度 

分 类 号：R969.1[医药卫生—药理学]