某地区麻疹病毒核蛋白及血凝素基因序列分析  

SEQUENCE ANALYSIS ON NUCLEOPOTEIN GENE AND HEMAGGLUTININ GENE OF MEASLES VIRUS ISOLATED IN SHENZHEN.

作　　者：何梅英[1] 陈伟红[1] 陈传德[1] 刘卫民[1] 卓菲[1] 

机构地区：[1]深圳市罗湖区疾病预防控制中心,深圳518020

出　　处：《现代预防医学》2005年第4期295-296,共2页Modern Preventive Medicine

基　　金：深圳市卫生科技立项 (2 0 0 2 0 41 6 6 )

摘　　要：目的:探讨深圳市麻疹病毒的基因型别和特征。方法:采用B95a细胞分离培养麻疹病毒,通过RT-PCR方法扩增麻疹病毒核蛋白N末端4 5 0bp片段和H基因合序列并测序、分析和基因库Genebank中麻疹的各代表株基因序列的同源性进行比较。结果:分离到1株麻疹病毒,N基因与H1基因型代表株相比同源性为98.4%,H基因与H1基因代表株同源性达99.5 %。Objective:To study the genetic character and genotype of measles in Shenzhen district.Methods:Throat swabs were collected for virus isolation with B95a cells. A 450 bp fragment of the C-terminal of the nucleoprotein(N) gene and hemagglutinin (H) gene were amplified by RT-PCR and subjected to sequence and phylogenetic analysis using DNASTAR software.Results:Only one strain was isolated from 15 throat-swabs, N genes homogeneity was 98.4% and H genes was 99.5% compared with other wild-type viruses of H1 genotype.Conclusion:Shenzhen circulating measles viruses were belong to clade H1.

关 键 词：麻疹病毒 基因序列分析 血凝素 PT-PCR方法 H1基因型 白及 细胞分离培养 同源性 代表株 基因型别 深圳市 H基因 N末端 核蛋白 基因库 N基因 流行株 

分 类 号：R373.11[医药卫生—病原生物学] R382.31[医药卫生—基础医学]