肾病患者血清荧光光谱分析初探  

Preliminary study on autofluorescence spectra of nephrotic serum

作　　者：吴敏[1] 丁建华[1] 李建东[1] 王传晶[2] 

机构地区：[1]大连理工大学物理系,大连116024 [2]中国人民解放军第210医院,大连116024

出　　处：《激光杂志》2005年第2期83-84,共2页Laser Journal

基　　金：国家自然科学基金资助项目(60278037)

摘　　要：利用波长为488.0nm的氩离子激光诱导血清的自体荧光,并对采集范围内的血清荧光光谱的峰值点位置及相对强度进行分析。发现,肾病患者血清的荧光峰位虽然较健康人的有红移趋势,但符合率不够高。而锌卟啉的荧光峰位( 5 90 .0nm)、原卟啉的荧光峰位( 63 0 .0nm)与峰值点的强度比能够将健康与病变血清很好的区分开,尤其是63 0 .0nm点与峰值点的强度比还能判别肾病变的程度。这一结果对于肾病的光谱诊断研究或许有一定的参考价值。An autofluorescence spectral method is proposed to analyze nephrotic serum.The autofluorescence spectra was excited by 488.0nm A+_rlaser and collected in the range of 495.0nm-640.0nm.Data processing was concentrated on both position of the peak value(PPV)in the spectra and the ratio of the intensity correspond to the peak value(RIP).The PPV of nephrotic serum shows a red-shift comparing with that of the health one,but it not always does so.However,when we pay more attention to the spectra between 590.0nm and 630.0nm,the data process of the RIP can get a quite clear separation between the nephrotic serum and health one.Especially,the RIP at 630.0nm can be used to quantify the stage of the nephritic.This preliminary data could be a valuable reference for the nephrotic diagnosis.

关 键 词：自体荧光 激光 肾病 血清 

分 类 号：R318.51[医药卫生—生物医学工程]