斑点金免疫渗滤法检测斯氏狸殖吸虫病患者血清IgG的研究  

DIGFA for Detecting Serum Antibody IgG in Cases Infected with Pagumogonimus skrjabini

作　　者：陈文碧[1] 张锡林[2] 王光西[1] 王敏[1] 杨兴友[1] 佘俊萍[1] 杨燕[1] 刘建军[1] 

机构地区：[1]泸州医学院人体寄生虫学教研室,四川泸州646000 [2]第三军医大学基础部病原生物学教研室,重庆400038

出　　处：《寄生虫病与感染性疾病》2004年第3期99-102,共4页Parasitoses and Infectious Diseases

基　　金：四川省教育厅科研重点资助项目 ( 2 0 0 3 A0 62 ) ;泸州医学院科研资助项目

摘　　要：目的　为寻求一种简便、快速、可靠的方法 ,用于诊断斯氏狸殖吸虫病。方法　以斯氏狸殖吸虫成虫可溶性抗原为包被抗原 ,胶体金标记羊抗人 Ig G为显色剂 ,建立检测斯氏狸殖吸虫病患者血清 Ig G的斑点金免疫渗滤法 ( DIGFA) ,并以 dot-ELISA作平行对照。结果　 DIGFA和 dot-ELISA分别检测 91例斯氏狸殖吸虫病患者血清 Ig G抗体 ,其阳性率分别为 96.7% ( 88/ 91 )和 92 .3 % ( 84/ 91 ) ,两法差异无显著性。DIGFA分别检测健康者血清 3 5例、日本血吸虫病患者血清 2 5例和华支睾吸虫病患者血清 2 2例 ,前者均为阴性 ,后两者的交叉反应率分别为 4.0 % ( 1 / 2 5 )和 4.5 % ( 1 / 2 2 ) ;DIGFA和 dot-ELISA两法的总符合率达 91 .7% ( 4 4/ 4 8)。结论　 DIGFA的敏感性和特异性与 dot-ELISA相近 ,但方法简便、快速 ,是一种检测斯氏狸殖吸虫病患者血清 Ig G抗体的较好的免疫诊断方法。Objective To develop a simple, rapid and reliable assay for diagnosis of pagumogonimiasis skrjabini. Method Using Pagumogonimus skrjabini adult worm antigen and IgG lebelled with colloidal gold as color developing agent, a dot immunogold filtration assay (DIGFA) for detecting pagumogonimiasis skrjabini antibody IgG was established and Dot-ELISA was used as parallel control. Results There were no significant differences in positive rates between DIGFA (96.7%,88/91) and Dot-ELISA (92.3%,84/91) in 91 proved cases. The negative rate of DIGFA in healthy persons reached 100%(35/35). The cross rates of DIGFA in schistosomiasis and clonorchiasis cases were 4.0%(1/25) and 4.5%(1/22). The coincidence rate of DIGFA and Dot-ELISA reached 91.7%(44/48). Conclusion DIGFA, an assay with simplicity and without special equipment, was as sensitive and specific as Dot-ELISA.

关 键 词：斯氏狸殖吸虫病 斑点金免疫渗滤法 dot—ELISA 

分 类 号：R532.22[医药卫生—内科学]