甲基紫6B共振光散射法测定脱氧核糖核酸  

DETERMINATION OF DEOXYRIBONUCLEIC ACID BY METHYL VIOLET 6B LIGHT-SCATTERING SPECTROSCOPY

作　　者：苏界殊[1] 陈小明[1] 罗和安[1] 龙云飞[2] 易慧勇[2] 

机构地区：[1]湘潭大学化学学院,湘潭411105 [2]湖南科技大学化学化工学院,湘潭411201

出　　处：《化学分析计量》2004年第5期12-14,共3页Chemical Analysis And Meterage

基　　金：湖南省自然科学基金资助项目 (0 0JJY2 0 84);湖南省教育厅资助项目 (0 3C61 1 )

摘　　要：基于甲基紫 6B与脱氧核糖核酸在酸性条件下共振光散射的增强效应 ,建立了测定DNA的共振光散射法。在 pH值为 2 .0～ 3.0的三羟甲基氨基甲烷 -盐酸缓冲溶液中 ,甲基紫 6B与fsDNA、ctDNA分子作用后共振光散射增强 ,其强度增加值与DNA的浓度呈线性关系 ,线性范围分别为 0～ 1.0、0～ 1.5mg/L ,相关系数分别为 0 .9993、0 .9997,检出限分别为 15 .3、12 .2 μg/L ,用于DNA合成样品的测定 ,测定结果的精密度、准确度较高。Resonance light-scattering (RLS) characteristics of interaction between methyl violet 6B and deoxyribonucleic acid (DNA) was studied in the medium with pH 2.36. The effects of interference substances and optimization of procedure were investigated and a new method was established for the determination of the DNA (fsDNA,ctDNA) at nanogram level based on RLS measurement. The linear range of the fsDNA is 0～1.0 mg/L and the ctDNA is 0～1.5 mg/L with the correlation coefficient of 0.999 3 and 0.999 7 respectively. The detection limit is 15.3 μg/L and 12.2 μg/L. The method has been applied to the determination of DNA in mixed samples with high accuracy and precision.

关 键 词：脱氧核糖核酸 共振光散射法 甲基紫 三羟甲基氨基甲烷 共振光散射增强 DNA分子 增强效应 酸性条件 缓冲溶液 线性关系 线性范围 相关系数 合成样品 测定结果 pH值 增加值 检出限 精密度 准确度 

分 类 号：O614.121[理学—无机化学] Q523[理学—化学]