Ac/Ds双元激活标签体系用于建立水稻突变体材料(英文)  

A Two-component Ac/Ds Activation Tagging System in Rice Genome for Mutant Development

作　　者：陈双燕[1] 吴运荣[1] 吴平[1] 

机构地区：[1]浙江大学生命科学学院,植物生理学与生物化学国家重点实验室,杭州310029

出　　处：《农业生物技术学报》2004年第4期369-373,共5页Journal of Agricultural Biotechnology

基　　金：Supported by the National High Science and Technology Program(863) of China.

摘　　要：将含有两个35S启动子和4个串联重复的35S增强子的Ac／Ds双元转座子标签载体转入水稻(O／yza sativa L．)基因组。100个转化植株的分析结果表明，Ds在T0植株的体细胞中切离频率高达60％。20个T1株系的600个T1植株的PCR分析结果表明，14个株系(70％)表现出较高的切离频率(约43％～100％)。Southem杂交发现在同一T1株系的不同植株中Ds转座既有相同的转座位点(约90％)，也有不同的转座位点(约10％)。A two-component Ac/Ds activation tagging system, with two 35S promoters and four tandem repeats of the enhancer fragment of this promoter within the Ds element, was introduced into rice (Oryza sativa L.) genome. The results from 100 T0 transgenic plants showed that the somatic excision frequencies of Ds were high to 60%. Fourteen out of 20 T1 plant lines showed excision frequencies between approximately 43% and 100%. Southern hybridization analysis revealed that the same bands and the different bands were observed between different plants of the same T1 line and the different bands were about 10% in all analyzed T1 plant lines.

关 键 词：水稻 体材料 突变 激活 35S启动子 分析结果 转座子标签 串联重复 转化植株 DS 增强子 基因组 体细胞 PCR 600 株系 频率 切离 位点 载体 

分 类 号：Q754[生物学—分子生物学] S511[农业科学—作物学]