三七复合有效成分对恒河猴细菌感染性子宫出血模型内膜组织一氧化氮及一氧化氮合酶的影响  

Influence of Two Kinds of Active Components from Panax Notoginseng on NO and iNOS in Inflammatory Metrorrhagia Model of Macaca mulatta

作　　者：尤昭玲[1] 马红霞[2] 王若光[1] 谈诊如[1] 林洁[1] 余建[1] 

机构地区：[1]湖南中医学院,湖南长沙410007 [2]广州中医药大学,广东广州510407

出　　处：《中国实验方剂学杂志》2004年第5期18-20,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金 (30 2 71 633);湖南省社会发展重大项目 (0 1SSY1 0 0 2 4)

摘　　要：目的 :探讨一氧化氮 (NO)及诱导型一氧化氮合酶 (iNOS)在炎性子宫出血中的作用及三七复合有效成分的影响。方法 :选择月经正常雌性育龄恒河猴 11只 ,随机分为中药组 (三七复合有效成分 ) 4只、模型组 4只及正常对照组 3只。应用化学比色法检测三组子宫内膜中一氧化氮及一氧化氮合酶的活性 ,用免疫组化法检测三组子宫内膜中一氧化氮合酶 (iNOS)蛋白的表达水平。结果 :中药组与模型组比较 ,子宫内膜NO、iNOS的活性及iNOS蛋白表达水平明显降低 ,差异有显著性 ,P <0 0 1;结论 :NO可能参与了炎性子宫出血的发病机制 ,三七减少炎性子宫出血的机制可能与调节NO。Objective:To explore the influence of two kinds of active components from Panax notoginseng on NO and iNOS on inflammatory Metrorrhagia model of Macaca multatta. Methods:Eleven normal menorrhea adult Rhesus monkeys were choosed as research object,and were divided into model group (four),TCM group(four) and Control group(three) at random.Colorimetric method were performed to estimate the activity of NO and iNOS in of model group,TCM group and normal control group,the immunohistochemical S\|p were performed to estimate the express level of iNOS in the endometrium of the three groups.Result:Compared to the model group,the activity of No and iNOS and the express level of iNOS in the endometrium of TCM gorup was obviously weaker,which are similar to that of the normal group.Conclusion:NO may be involred in the pathogenesis of inflammatory Metrorrhagia.Two kinds of active components from Panax notoginseng reduce inflammatory Metrorrhagia by regulating the activities of NO and iNOS and the express level of iNOS in the endometrium.

关 键 词：子宫出血 一氧化氮 一氧化氮合酶 三七 恒河猴 

分 类 号：R285.5[医药卫生—中药学]