萝卜雄性不育性的几种特性研究  

Studies on Several Properties of Radish Male-sterility

作　　者：周长久[1] 张友良[1] 

机构地区：[1]北京农业大学园艺系

出　　处：《园艺学报》1994年第1期65-70,共6页Acta Horticulturae Sinica

摘　　要：通过对萝卜（ＲａｐａｎｕｓｓａｔｉｖｕｓＬ．）２３４株雄性不育株的花器形态观察，将其分成３种类型：花冠退化型，雄蕊萎缩型，花粉败育型。花粉败育型中按柱头外露情形可观察到柱头外露，半外露及不外露３种状态。萝卜雄性不育系的四分体小孢子正常，花粉粒从小孢子形成以后开始退化。４０１Ａ、７７０１Ａ在小群体中的不育株率达１００％。通过３对雄性不育材料及其保持系的酯酶同功酶分析，不育系与保持系之间的区别主要表现在第５条和第６条酶带，酶带显色深浅及酶带数目有明显差异。不育系４０１Ａ与其保持系４０１Ｂ的叶绿体ＤＮＡ的限制性核酸内切酶ＥｃｏＲＩ、ＢａｍＨＩ消化后的电泳图谱表明，不育系与保持系的ｃｔＤＮＡ之间存在差异。用ＳＤＳ－ＰＡＧＥ方法分析了不育系４０１Ａ、７７０１Ａ及其保持系的叶绿体类囊体膜多肚，表明第５及第１０条多肽存在差异。According to the performance of nower organ radish (Raphanus sativus L.) male-sterile plants can be classified into three types: 1. form of degenerative corolla; 2. form of shrivelling stamen; 3. form of abortive pollen. As far as the stigma is concerned,the form of abortive pollen includes emerged stigma, half-emerged stigma and nonemerged stigma forms. Most of radish male- sterile plants belong to form of abortive pollen,half-emerged stigma.The analysis of esterase isozymes of flower buds of N (normal) plants 48B, 7701B,401B and MS plants 48A, 7701A, 401A by vertical polyacrylamide gel electrophoresis showed that the difference between N and MS in the isozymes zymogram bands was remarkable, and that the esterase isozymes of N were more active than those of MS.The difference ill the SDS- PAGE patterns of thylakoid membrane polypeptides between N and MS was also observed. Chloroplast DNA of normal 401B and MS 401A were compared on the basis of restriction endonuclease analysis. Chloroplast DNA isolated from normal 401 B and MS 401A had different restriction patterns with EcoRI and BamHI.

关 键 词：萝卜 雄性不育性 叶绿体DNA 

分 类 号：S631.103[农业科学—蔬菜学]