三种不同的方法检测HBsAg的比较  

3 Methods for Detecting HBsAg and the Difference of Them

作　　者：胡晓武[1] 刘伶[2,3] 

机构地区：[1]安徽省马鞍山市人民医院检验科,243000 [2]安徽省马鞍山市人民医院检验科【关键词】荧光定量PCR [3]电化学发光,243000

出　　处：《实用全科医学》2005年第3期265-265,267,共2页Applied Journal Of General Practice

摘　　要：目的探讨三种检验方法间的关系及临床价值。方法对用ELISA法测出HBsAg阳性的标本,再用荧光定量PCR(FQ-PCR)和电化学发光(ECL)的方法测其拷贝数和含量。结果对用ELISA法“大三阳”的40份标本中,荧光定量PCR阳性率为85%,电化学发光的方法阳性率为100%;ELISA法“小三阳”的60份标本中,荧光定量PCR阳性率为8.3%,电化学发光的方法阳性率为98%。结论三种检验方法对临床有不同的指导意义。Objective To investigate the relationship of three methods (ELISA, FQ-PCR and ECL) and the clinical significances of them. Methods 100 HBsAg positive samples (40 samples were positive for HBsAg, HBeAg, and HBcAb and 60 samples were positive for HBsAg , HBeAb, and HBcAb), which detected by ELISA, were selected for this study. All samples were detected by fluorescence quantitative polymerase chain reaction assay (FQ-PCR) and Electrochemiluminescence (ECL) technology again. Results In 40 positive samples for HBsAg, HBeAg and HbcAb, the positive rate of FQ-PCR was 85% and the positive of ECL was 100%. In other 60 samples, the positive rates of FQ-PCR and ECL were 8.3% and 98% respectively. Conclusions There were statistical difference and discrepant results among three methods for detecting HBsAg in serum.

关 键 词：荧光定量PCR ELISA法 电化学发光 HBSAG阳性 检测 ELISA法 检验方法 “小三阳” 阳性率 临床价值 PCR) 指导意义 标本 拷贝数 大三阳 

分 类 号：R512.62[医药卫生—内科学] R446.61[医药卫生—临床医学]