芦丁-α-鼠李糖苷酶基因的克隆  

Cloning of rutin-α-rhamnosidase gene

作　　者：马安周[1] 王栩林 鱼红闪[1] 金凤燮[1] 

机构地区：[1]大连轻工业学院生物与食品工程学院,辽宁大连116034 [2]大连市产品质量监督检验所,辽宁大连116021

出　　处：《大连轻工业学院学报》2005年第1期8-11,共4页Journal of Dalian Institute of Light Industry

基　　金：国家自然科学基金资助项目(20076017).

摘　　要：根据芦丁 α 鼠李糖苷酶N端氨基酸序列设计简并引物,从Absidiasp.R9g的总RNA出发,通过逆转录 聚合酶链反应(RT PCR)扩增出芦丁 α 鼠李糖苷酶基因片段。将RT PCR获得的1条1.6kb的目的条带与pMD18 T载体连接,转化大肠杆菌JM109感受态细胞。酶切鉴定结果表明目的片段克隆成功。RT-PCR was carried out with degenerate primers designed according to the N-terminal amino acid sequences of rutin-α-rhamnosidase. A 1.6?kb cDNA fragment was amplified from the total RNA of Absidia sp.R9g and ligated with vector pMD18-T.E.coli JM109 competent cells were transformed with the recombinant plasmid. The result shows that clones are successful.

关 键 词：芦丁-α-鼠李糖苷酶 RT-PCR 克隆 基因 

分 类 号：Q785[生物学—分子生物学]