对甜菜夜蛾高毒力Bt新菌株的PCR-RFLP分析及生物活性测定  

PCR-RFLP analysis of new Bt strains against Spodoptera exigua and toxicity activity analysing

作　　者：程林友[1] 陆秀君[1] 李国勋[1] 

机构地区：[1]河北农业大学植物保护学院,河北保定071001

出　　处：《东北农业大学学报》2005年第2期161-165,共5页Journal of Northeast Agricultural University

基　　金：国家973项目(2003CB114201)资助

摘　　要：利用PCR-RFLP对Bt.新菌株cyz-13,T4-3的基因型进行分析。结果表明,cyz-13含有cry1Aa,cry1Ac,cry1Bc,cry2Ab4种基因型,其中cyz-13菌株的PCR产物的酶切片断类型不同于已知的基因类型;T4-3菌株含有cry1Aa,cry1Ac,cry1Ag,cry2Ac,cry1Ia5种基因型。室内生测结果显示,两菌株对甜菜夜蛾、粘虫、棉铃虫、玉米螟均具有高毒力,好于H D-1标准菌株。两菌株的发酵液均有杀虫活性,二者上清的杀虫因子不同。PCR-RFLP was used for detecting the cry-type genes from new Bacillus thuringiensis strains, T4-3 and cyz-13. The results showed that there was five cry-type genes including cry1Aa, cry1Ac, cry1Ag, cry2Ac and cry1Ia in Bt T4-3 strains; Three types of gene of cry1Ac cry1Bc cry2Ab were identified in Bt cyz-13 strain, The result of restriction enzyme analysis showed that the fragments from cyz-13 strain was different from all of published genes the bioassay showed that there was more infectivity with T4-3 or cyz-13 compated to standared strain, HD-1.

关 键 词：苏云金杆菌 CRY基因 PCR—RFLP分析:生物测定 

分 类 号：S476[农业科学—农业昆虫与害虫防治] Q-33[农业科学—植物保护]