草地早熟禾不同品种干旱胁迫下HVA1抗旱基因表达分析  

Expression analysis of drought resistance HVA1 gene under drought stress in different Poa pratensis cultivars

作　　者：陈雅君[1] 王洪宝[2] 冯淑华[3] 李炜[4] 

机构地区：[1]东北农业大学园艺学院,黑龙江哈尔滨150030 [2]东北农业大学动物科技学院,黑龙江哈尔滨150030 [3]黑龙江农业工程职业学院,黑龙江哈尔滨150088 [4]黑龙江省农业科学院,黑龙江哈尔滨150086

出　　处：《东北农业大学学报》2005年第2期166-169,共4页Journal of Northeast Agricultural University

基　　金：农业部草地资源生态重点开放实验室2004年度资助;黑龙江省自然科学基金资助(C0207)。

摘　　要：根据大麦(Hordeum valgare)HVA1抗旱基因(X78205)的mRNA序列设计引物,提取干旱胁迫下草地早熟禾叶片中的RNA,以反转录后的cDNA为模板,进行PCR反应,扩增产物经回收、连接、转化和酶切鉴定为阳性重组子,其序列长度为324bp。经比较,与大麦HVA1基因序列的同源性达到79.27%,表明所克隆的基因序列为草地早熟禾HVA1基因部分序列。Total RNA in leaves of Poa pratensis cultivars under drought stress was extracted for reversing transcription into cDNA and then the cDNA was as a template for PCR reaction according to designing primer of cds of Hordeum valgare HVA1 drought resistance gene(X78205). The amplified products were positive recon identified by using procedures of recovery, connection, transformation and enzyme separation. The length of cloned gene sequence was 324 bp identity reached 79.27% with Barley HVA1 gene that means the cloned gene sequence was the partial HVA1 gene of Poa pratensis.

关 键 词：草地早熟禾 干旱胁迫 HVA 1抗旱基因 

分 类 号：S688.4[农业科学—观赏园艺] Q945.78[农业科学—园艺学]