龙骨星蕨与膜叶星蕨的关系:来自叶绿体rbcL、rps4和trnL-trnF、rps4-trnS序列的证据  

Relationship of Microsorium membranaceum var. carinatum and Microsorium membranaceum var. membranaceum based on the sequence analysis of chloroplast rbcL , rps4 , trnL-trnF and rps4-trnS

作　　者：李春香[1] 陆树刚[2] 

机构地区：[1]中国科学院南京地质古生物研究所现代古生物学和地层学国家重点实验室,南京210008 [2]云南大学生态学与地植物学研究所,昆明650091

出　　处：《生物多样性》2005年第2期174-179,共6页Biodiversity Science

基　　金：中国科学院知识创新工程重要方向项目(KZCX2 SW 130);国家自然科学基金(40302003; 30370116)

摘　　要：龙骨星蕨(Microsoriummembranaceumvar. carinatum)是新近发表的一个变种,从形态特征看,它与原变种膜叶星蕨(M. membranaceumvar. membranaceum)可以很容易地区分。但将龙骨星蕨与另外 3种国产星蕨属 (Mi crosorium)植物叶绿体基因组中rbcL、rps4基因和trnL trnF、rps4 trnS基因间隔区进行PCR扩增和序列分析,并与已经发表的真蕨类的相应序列进行比较发现,龙骨星蕨与膜叶星蕨在所研究的 4个DNA片段中均未表现出序列差异(除了trnL trnF区的一个 2bp长度差异外 ),而所比较的其他真蕨类变种间的相应DNA片段均存在一定的差异。因此DNA序列证据不支持龙骨星蕨变种的成立。Microsorium membranaceum var. carinatum , a newly published variety, can be distinguished easily from the original variety M. membranaceum var. membranaceum by the stipe morphology. The cross section of stipes is triangular in the former, but semicircular in the latter. Here phylogenetic relationship of the two varieties was investigated based on the sequence analysis of chloroplast rbcL , rps4 , trnL trnF , and rps4 trnS intergenic spacers. Furthermore, the published relevant sequences from other ferns were also analyzed for the comparison of genetic distance. As a result, no sequence difference in the studied four cpDNA regions was found between M. membranaceum var. carinatum and M. membranaceum var. membranaceum , but some difference in the corresponding DNA fragments is present among other fern varieties. Hence, the status of Microsorium membranaceum var. carinatum as a distinct variety is not supported by the DNA analysis.

关 键 词：RBCL 龙骨 证据 DNA片段 膜 叶绿体基因组 PCR扩增 基因间隔区 DNA序列 形态特征 序列分析 序列差异 原变种 蕨类 植物 

分 类 号：Q948[生物学—植物学]