日本结缕草植株再生体系的研究  

Studies on plant regeneration system of Zoysia japonica from mature seed

作　　者：张俊卫[1] 唐蜻[1] 包满珠[1] 

机构地区：[1]园艺植物生物学教育部重点实验室华中农业大学园艺林学学院,湖北武汉430070

出　　处：《草业学报》2005年第2期48-51,F003,共5页Acta Prataculturae Sinica

基　　金：863计划资助项目(2001AA244051)资助。

摘　　要：以日本结缕草的成熟胚为外植体,对其组织培养体系进行了优化.试验结果表明,1)培养基中添加脯氨酸能提高愈伤组织诱导率,而添加BA能降低愈伤组织的诱导率,低浓度的NAA(0.1～0.2 mg/L)能促进愈伤组织的形成,愈伤组织诱导的最佳培养基为ND+2,4-D 3.0 mg/L+NAA 0.2 mg/L+L-Pro 500 mg/L,愈伤组织诱导率达57.3%;2)愈伤组织继代的最佳培养基为MSD+2,4-D 1.0 mg/L;3)愈伤组织分化的最佳培养基为MSD+NAA 0.3 mg/L+KT 0.5 mg/L+BA 0.05 mg/L,分化率达36.7%.Taking mature caryopses as explant, the plant regeneration system of Zoysia japonica was optimized. The results are as follows:1) The addition of L-Proline or lower concentration NAA(0.1-0.2 mg/L) to the media increases callus induction rate, the addition of BA to the media decreases callus induction rate, the best media for callus induction was ND+2,4-D 3.0 mg/L+NAA 0.2 mg/L and the ratio of callus induction was (57.3%); 2)The best media for callus subculture was MSD+ 2,4-D 1.0 mg/L; 3)The best differentiation media was MSD+NAA 0.3 mg/L+ KT 0.5 mg/L+ BA 0.05 mg/L and the ratio of differentiation was 36.7%.

关 键 词：日本结缕草 成熟胚 植株再生 愈伤组织 

分 类 号：S688.4[农业科学—观赏园艺]