利用RAPD标记分析狼尾草属牧草品种间的遗传关系  

Analysis of genetic relationships of cultivars in Pennisetum by RAPD markers

作　　者：解新明[1] 卢小良[1] 

机构地区：[1]华南农业大学南方草业中心,广东广州510642

出　　处：《草业学报》2005年第2期52-56,共5页Acta Prataculturae Sinica

基　　金：农业部948项目(202102);华南农业大学博士科研启动基金(2004B006)资助。

摘　　要：采用RAPD分子标记技术对狼尾草属8个品种(品系)间的遗传关系进行了分析,结果发现,19 个引物共扩增出169条带,其中111条带为多态性带,占总带数的65.7%,表明在这8 个品种(品系)间具有较高的遗传多样性,存在有一定的遗传分化。从UPGMA聚类分析的结果来看,象草、象草新品系、矮象草、N51、王草和桂牧1 号作为一个谱系群的bootstrap值为73%,反映了它们之间较为密切的亲缘关系;同时发现,杂交狼尾草与其他7 个草种间存在有较明显的遗传分化,表明Pennisetum americanum 遗传成分的多少及有无在这8 个品种(品系)间遗传关系的调节中起着重要的作用。另外,相对于其他6 个草种,象草新品系与矮象草具有最小的遗传距离(0.130),从而为象草新品系与矮象草之间亲缘关系的确定提供了重要证据。The genetic relationships of eight cultivars and lines in Pennisetum. were analyzed using random amplified polymorphic DNA (RAPD) markers. A total of 169 reproducible RAPD fragments were produced using 19 random primers. One hundred and eleven fragments (65.7% of the total observed) were polymorphic, which indicated that there was high genetic diversity among these eight genotypes. From the results of UPGMA cluster analysis, ‘Huanan’, ‘New line’ , ‘Mott’, ‘N (51)’, ‘Reyan NO.4’ and ‘Guimu NO.1’ formed a pedigree group, which was strongly supported by bootstrap analysis. There was also genetic differentiation between the ‘Hybrid pennisetum’ and the other seven genotypes. In addition, the ‘New line’ of elephantgrass was most similar to ‘Mott’ (genetic distance of 0.130), compared with other six cultivars.

关 键 词：狼尾草属 品种 RAPD 遗传关系 

分 类 号：S543.903[农业科学—作物学]